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Promoter induced by plant system acquired character resistance inducer and its application

A promoter, plant technology, applied in the field of plant molecular biology and genetic engineering

Inactive Publication Date: 2004-10-20
FUDAN UNIV
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  • Summary
  • Abstract
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Problems solved by technology

[0019] However, so far, there has been no report on PBZ-induced enhancement of the expression of the promoters of related genes, and there is no report of an inducible system that regulates the corresponding promoter and structural gene combination in transgenic plants.

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0029] 1. Rice cultivation, PBZ treatment, material collection and identification of disease resistance

[0030] Take an appropriate amount of rice seeds, soak them in tap water for a while, and remove the shriveled grains. Then sterilized with 3% hydrogen peroxide solution for 30 minutes, then discarded the hydrogen peroxide, washed several times with sterile tap water and soaked the seeds in sterile tap water for 3 days at 30°C. After the seeds are white, they are evenly spread on an enamel plate lined with 4 layers of sterile gauze, and cultivated for 7 days at 27°C under a cycle of 16 hours of light / 8 hours of darkness. After that, the rice was treated with 100 ppm allylisothiazole on days 1-9 respectively, and the treatment method was soaking in the root solution. After the treatment, all inoculate Magnaporthe oryzae. The inoculation method is: put in the inoculation box, spray inoculation under the condition of 26° C. and relative humidity of more than 85%, and the conc...

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Abstract

A promotor induced by the acquired resistance inducter of plant system and its application are disclosed. The promoter comes from the gene of Arabidoposis, which is sensitive to PB2 and its relative compound. The nucleic acid sequence of desired target product gene is combined with said promotor fragment to obtain recombinant DNA, which is used to transform plant to obtain a new transgenic plant, in which the expression of target gene in recombinant DNA is regulated by the chemical inducing of PBZ.

Description

technical field [0001] The invention belongs to the technical field of plant molecular biology and genetic engineering, and specifically relates to a promoter induced by a plant system acquired resistance inducer and application thereof. The promoter is derived from the gene responsive to the induction of allylisothiazole (PBZ, Probenazole, 3-allyloxy-2-phenylpropenazole-1,1-dioxide) and its related active products in Arabidopsis Upstream untranscribed region sequence. This promoter fragment is capable of initiating and driving the expression of downstream nearby genes with the aid of an inducer in both monocot and dicot plants. By using the promoter fragment to construct a recombinant expression vector to transform plants, new transgenic plants can be obtained. In these novel transgenic plants, PBZ and its related active product (BIT) can induce the expression and regulation of target genes at will. Background technique [0002] In the past ten years, people have obtaine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H5/00C12N15/11C12N15/29C12N15/67C12N15/82
Inventor 蒯本科赵慧芳顾华王喜萍赵向辉
Owner FUDAN UNIV
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