Method for breeding cabbaye type rape non-deficiency of non-greensick turnip cytoplasm male sterility line
A technology of Brassica napus and male sterile lines, which is applied in the direction of botany equipment and methods, applications, plant gene improvement, etc., can solve the problems of affecting insect pollination, pollen, and maintaining narrow sources, so as to improve production yield and seedling stage. Strong growth and the effect of increasing the amount of seed production
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[0021] 1) In 1996, M05000 air-dried seeds of the cytoplasmic male sterile line (Ogu cms) of Brassica napus radish with chlorotic leaves and dysplasia of nectary glands were selected. 60 Co-γ rays were irradiated as the irradiation source, and the irradiation dose was 2800Gy, and the first-generation seeds M 1 ;
[0022] 2) From 1996 to 1997, M 1 Carry out sowing, select 60 plants with light radiation damage, that is, plants that are relatively close to normal, and carry out hybridization with the pollen of Zhongshuang No. 2 to obtain the second generation seed M 2 ; At the same time, Zhongshuang 2 was self-crossed as the backcross parent;
[0023] 3) From 1997 to 1998, M 2 Sowing is carried out, and the pollen of the single plant Zhongshuang No. 2 is selected in which the plant is not short of green, and the pollen of the third generation seed M is obtained. 3 ; At the same time, Zhongshuang 2 was self-crossed as the backcross parent;
[0024] 4) From 1998 to 1999, M 3 C...
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