Method for breeding cabbaye type rape non-deficiency of non-greensick turnip cytoplasm male sterility line
A technology of Brassica napus and male sterile lines, which is applied in the direction of botany equipment and methods, application, plant gene improvement, etc., can solve the problems of affecting insect pollination, pollen, and maintaining narrow sources, so as to increase production yield and improve production efficiency. The effect of seed quantity and seedling growth is strong
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[0021] 1) In 1996, air-dried seeds M of the cytoplasmic male sterile cytoplasmic male sterile line (Ogu cms) of Brassica napus with lack of green leaves and dysplasia of nectaries were selected. 0 5000 capsules, use 60 Co-γ ray is used as the irradiation source for irradiation, the irradiation dose is 2800Gy, and the first generation seed M is obtained 1 ;
[0022] 2) From 1996 to 1997, M 1 For sowing, select 60 plants with light radiation damage, that is, plants with performance closer to normal, and cross pollen with Zhongshuang No. 2 to obtain the second generation seed M 2 ; At the same time, self-crossing on Zhongshuang No. 2 is used as the back-crossing parent;
[0023] 3) In 1997-1998, M 2 Sowing, select the pollen of the single plant Suizhongshuang No. 2 whose plants are not lacking in greenness for backcrossing to obtain the third generation seed M 3 ; At the same time, self-crossing on Zhongshuang No. 2 is used as the back-crossing parent;
[0024] 4) In 1998-1999, M 3 A...
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