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Method for breeding cabbaye type rape non-deficiency of non-greensick turnip cytoplasm male sterility line

A technology of Brassica napus and male sterile lines, which is applied in the direction of botany equipment and methods, application, plant gene improvement, etc., can solve the problems of affecting insect pollination, pollen, and maintaining narrow sources, so as to increase production yield and improve production efficiency. The effect of seed quantity and seedling growth is strong

Inactive Publication Date: 2005-09-14
ZHEJIANG UNIV
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  • Application Information

AI Technical Summary

Problems solved by technology

("Breeding and Utilization of Hybrid Rapeseed" Hubei Science and Technology Press 1995) the promotion of these sterile types in rapeseed hybridization has achieved great economic and social benefits, but there are also many weak points: (1) temperature Sensitive, incomplete sterility, which seriously affects the yield advantage of hybrid rapeseed; for example, the temperature has been seriously abnormal in recent years, because the hybrid rapeseed used in my country's production is all Porima and Shan 2A cytoplasmic male sterile types, at low temperature Under normal circumstances, a large amount of micro pollen generally appears in these sterile lines, which makes the quality of hybrid rapeseed production in various provinces and cities drop sharply, causing huge economic losses to agricultural production
(2) The maintenance source is narrow, and it is more difficult to find excellent maintenance lines, so it is difficult to obtain strong dominant combinations and new combination updates
[0004] Although Brassica napus Ogura radish cytoplasmic male sterile (Ogura cms) has the above-mentioned advantages, the material is severely lacking in chlorosis at low temperature (temperature ≤ 12°C), and the whole plant of the seedlings in the field is yellow; the nectaries are stunted, Interfering with insect pollination; these disadvantages made this CMS line of Brassica napus ultimately unusable in production

Method used

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Embodiment 1

[0021] 1) In 1996, air-dried seeds M of the cytoplasmic male sterile cytoplasmic male sterile line (Ogu cms) of Brassica napus with lack of green leaves and dysplasia of nectaries were selected. 0 5000 capsules, use 60 Co-γ ray is used as the irradiation source for irradiation, the irradiation dose is 2800Gy, and the first generation seed M is obtained 1 ;

[0022] 2) From 1996 to 1997, M 1 For sowing, select 60 plants with light radiation damage, that is, plants with performance closer to normal, and cross pollen with Zhongshuang No. 2 to obtain the second generation seed M 2 ; At the same time, self-crossing on Zhongshuang No. 2 is used as the back-crossing parent;

[0023] 3) In 1997-1998, M 2 Sowing, select the pollen of the single plant Suizhongshuang No. 2 whose plants are not lacking in greenness for backcrossing to obtain the third generation seed M 3 ; At the same time, self-crossing on Zhongshuang No. 2 is used as the back-crossing parent;

[0024] 4) In 1998-1999, M 3 A...

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PUM

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Abstract

The present invention discloses a method for breeding cabbage rape non-chlorisis radish cytoplasm male sterility line, and is characterized by that it adopts 60 Co-gamma as radiation source to treat the cabbage rape radish cytoplasm male sterility type cytoplasm mate sterility line (Ogu cms) seed whose leaf has chlorisis and nectary development is poor, and makes the non-chlorisis and nectary development is poor, and makes the non-chlorisis and nectary development is poor, and makes the non-chlorisis mutated plant produced by Ogu cms seed and non-chlorisis conventional rape seed undergo the processes of hybridization and backcross breeding so as to obtain the invented product with normal leaf green, good nectary development and stable sterility.

Description

Technical field: [0001] The invention belongs to a method for breeding cytoplasmic male sterile lines of radish, more specifically a method for breeding non-green radish cytoplasmic male sterile lines of Brassica napus. Background technique: [0002] Rapeseed is the main oil crop in my country. In the production and utilization of rapeseed heterosis, my country has discovered excellent Polima (POL cms Fu Tingdong 1981) and Shaan 2A (Li Dianrong 1980) cytoplasmic male sterility types. ("Hybrid Rapeseed Breeding and Utilization" Hubei Science and Technology Press, 1995) The promotion of these sterile types in rape hybridization has achieved great economic and social benefits, but there are also many shortcomings: (1) Temperature Sensitive and incomplete sterility, which seriously affects the yield advantage of hybrid rape; for example, the temperature has been severely abnormal in recent years, because the hybrid rapes used in production in China are all Polyma and Shaan 2A cytopla...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/06
Inventor 张明龙
Owner ZHEJIANG UNIV
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