Plant root excretion continuous collection method and apparatus

Abstract

A plant root excretion continuous collection method and apparatus, wherein the plant is placed in the cultivation dish equipped with quartz sand, the bottom opening of the cultivation dish is connected to a glass bottle containing resin through pipe lines, an opening is arranged at the bottom of the glass bottle, then connected to the cultivation dish through pipe lines, forming a circulated system, adding different solvents into the circulating pipes, for the three dissimilar collected root secretion, i.e., neutral, acidic or basic, making the root secretion absorbed on the surface of the resin when flowing through the resin along with the solvent of the circulatory flow, after a specific period of time, perform elution, compression and filtration to the root secretion, finally conduct measuring.

Description

1. Technical field [0001] The invention relates to the collection of plant root exudates, in particular to a method for continuously collecting plant root exudates and a device thereof, belonging to the fields of soil, microorganism and plant science. 2. Background technology [0002] In addition to the shed cells and the mucus produced, plant roots also secrete a wide variety of soluble substances, such as sugars with different molecular weights, organic acids, amino acids, fatty acids, steroids, enzymes, auxins, etc., mainly Sugars, organic acids and amino acids. Root exudates vary widely by plant species, physiological factors (e.g., age, nutritional status) and abiotic conditions (e.g., temperature, soil structure, aeration, and moisture content), and are also related to microbial activity (amount consumed), from root The amount that is secreted and then enters the root is related to the damage degree of the root, and if it is collected in the soil, it is related to the...

AI Technical Summary

Problems solved by technology

[0003] Today, however, our knowledge of rhizosphere chemistry is extremely limited, mainly because researchers often face the following three problems when studying rhizosphere exudates: The exudates to be collected are usually very low in content, requiring a large number of plants and prolong the collection time to obtain a sufficient amount of secretions; the collection and processing of samples is very cumbersome, and some unstable compounds will be denatured; when the traditional solvent extraction method collects trace secretions, growth substrates, containers, nutrient solutions, water , extractant, etc. will cause serious contamination to the sample

Although the rhizosphere soil solution sampler can directly extract the soil solution in situ, its PVC pipe contains 30% plasticizer and a small amount of stabilizer, and a trace amount of sample can enter the sample. In addition, the taken solution often contains a large amount of Soil organic matter, so it will also cause greater pollution and interference to the sample

In addition, soil is a very complex multiphase system, and the influencing factors of soil solution chemistry have not been clarified. In addition, there are temporal and spatial variations in soil, which adds many difficulties to the collection of representative soil solutions, making it difficult to collect A solution representative of real root exudates

Images