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Immune chromatographic paper strip and method for quick detecting pathogen and toxin in food

A technology of pathogenic bacteria and strip separation, applied in the field of rapid detection of common pathogenic bacteria or toxins in food, can solve the problems of cumbersome, single binding site, chemical pollution, etc., and achieve the effect of rapid detection and simple detection operation.

Inactive Publication Date: 2003-11-26
INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Foreign chromatography strips for detecting bacteria use monoclonal antibodies, and pretreatment (lysing) is required before testing target samples, which is cumbersome
Although the monoclonal antibody technology has strong specificity, due to the single binding site, two antibodies targeting different epitopes of the antigen need to be used; in addition, the structure of the bacterial antigen is complex, and the steric hindrance makes it difficult for the monoclonal antibody labeled with colloidal gold to bind to the bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0023] The preparation of the colloidal gold-labeled immune paper chromatography strip of the present invention first collects common pathogenic bacteria or toxins in food, i.e. Salmonella, Shigella, Staphylococcus aureus, botulinum, Vibrio parahaemolyticus, pathogenic Escherichia coli, Proteus, Yersinia enterocolitica, Listeria monocytogenes, Vibrio cholerae, Streptococcus faecalis, Escherichia coli O157:H7, staphylococcal enterotoxin, botulinum toxin, aflatoxin, etc., For the preparation of antigens, use the above antigens to immunize white rabbits for the preparation of antibodies, and then purify the antibodies for the preparation of immune colloidal gold, combine immune colloidal gold with probe conjugate pads, common pathogenic bacteria or toxin colloids in food The gold probe conjugate pad is placed on one end of the nitrocellulose membrane, and the other end is provided with a water-absorbing plate pad. The control line, the bacteria or toxin antibody detection line are...

Embodiment 1

[0025] Example 1: Detection of Artificially Contaminated Salmonella Food

[0026] Cut rice, steamed buns cut into slices, and sauced pork liver, ham sausage, pork, beef, mutton, chicken, and duck raw and cooked 50g each in the market, cut into small pieces, put them into a clean conical flask, add 10 3 / ml Salmonella typhimurium (strain number 50020 was purchased from the National Culture Collection Center of the Ministry of Health) 10ml, cultivated for a certain period of time. Take 10g of the solid sample and put it into another Erlenmeyer flask, add 90ml of normal saline, shake vigorously, let it stand for a while, and take the supernatant for testing; take 1ml of milk and dilute it with 9ml of normal saline for testing directly. The total number of bacteria in the sample solution was counted by the agar count method. The detection method is: place the immunochromatographic test strip in the sample to be tested (the height of the placement should not exceed the colloidal ...

Embodiment 2

[0027] Embodiment 2: Detection of different food sample preparation methods

[0028] Cut 100g of rice, steamed buns, and sauced pork into small pieces, put them into a clean Erlenmeyer flask, add 10 3cfu / ml Salmonella type A (the strain number is 50004 purchased from the National Culture Collection Center of the Ministry of Health) 10ml, cultivated for a certain period of time. Take 10g of each sample and put them into conical flasks containing 90ml of normal saline, 0.01mol / 1 pH7.4 phosphate buffer, tap water, and distilled water, shake vigorously, let stand for a while, and take the supernatant for detection , Count the total number of bacteria in each diluted sample solution by agar counting method. The detection method is: place the immunochromatographic test strip in the sample to be tested (the height of the placement should not exceed the colloidal gold conjugate pad in the test strip), and when the sample liquid swims to the absorption pad, take out the Salmonella Im...

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PUM

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Abstract

The present invention discloses a kind of immunogical chromatographic paper strip for sensitive, specific and fast detection of common pathogen and toxin in food and the detection method. The present invention is the new technology for detecting pathogen and toxin in food and its application. Now, there are great amount of reports on applying colloidal gold immunogical chromatographic test paper in detecting soluble antigen and bacteria as granular antigen are detected after being treated via extraction or other processes. The present invention features that special chromatographic film and optimized conditions are adopted so that the immunogical chromatographic paper strip may be used directly in detecting bacteria. The present invention may be used in the fast detection of common pathogen in food and the fast diagnosis of toxin in food.

Description

technical field [0001] The present invention relates to the rapid detection of common pathogenic bacteria or toxins in food, more specifically, it relates to an immune paper chromatography strip and its detection method for rapidly detecting common pathogenic bacteria or toxins in food by using colloidal gold and immune chromatography technology . Background technique [0002] "Food is paramount to the people." Human beings cannot live without food. Food is one of the most important commodities in any society's economy. Therefore, the problem of food safety is concerned by millions of people. But the current food safety problem is not optimistic. In today's world, disease due to contaminated food is a significant contributor to declining living standards, declining health and economic productivity. Food poisoning has become one of the most common social, economic and environmental problems. According to statistics, in 1980 alone, in developin...

Claims

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Application Information

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IPC IPC(8): G01N33/02G01N33/53G01N33/543G01N33/558G01N33/569
Inventor 李君文宋农王新为郑金来古长庆金敏晁福寰
Owner INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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