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Human red blood cell source depressor factor and use thereof

A technology of erythrocytes and factors, applied in medicine, field of erythrocyte-derived antihypertensive factor

Inactive Publication Date: 2003-10-22
THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
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Problems solved by technology

Since their interest was focused on the study of HF, there have been no reports of this laboratory and others continuing to study AHF

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  • Human red blood cell source depressor factor and use thereof

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Embodiment 1

[0047] Take 400ml of human blood (collected from Peking Union Medical College Hospital Blood Bank, which contains 1% (v / v, 10mg / ml) heparin) in a refrigerated high-speed centrifuge (Hitachi Kokt Co., Ltd.) at 4 ° C at 2000g for 15 After 1 minute, the plasma and the upper fluffy substance were discarded to obtain red blood cells; about 4000ml deionized water was added to the obtained red blood cells to hemolyze them; 15 minutes, and then cooled in an ice bath; the obtained product was centrifuged at 12,000g at 4°C for 20 minutes in a centrifuge, and the supernatant was retained; the supernatant was ultrafiltered with an ultrafiltration membrane of Amico Division, and the collected molecular weight was less than 500. The ultrafiltrate of Dun; The ultrafiltrate that obtains is carried out freeze-drying; The concentration of described freeze-drying sample is made into 0.25g / ml with deionized water and carries out column chromatography fractionation, wherein the specification of col...

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Abstract

The present invention relates to an erythrocyte duoparental depressor factor (EDDF), its preparation method and application. Its preparation method includes the following steps: using human blood andadopting centrifugal separation process to obtain erythrocyte, using deionized water to hemolyze erythrocyte, after the hemolysate is stood still at 4 deg.C, heating the hemolysate at water bath with100 deg.C, then cooling in ice bath, centrifugalizing obtained product more than 12000g at 4 deg.C to take supernatant, ultrafiltering supernatant to collect the ultrafiltrate whose molecular weight is less than 500D, freeze-drying said ultrafiltrate, making the freeze-dried sample undergo the process of column chromatograph for sorting and separation so as to obtain the invented product which can be used for preparing medicine for curing hypertension disease.

Description

technical field [0001] The present invention relates to an endogenous antihypertensive factor with antihypertensive effect, more specifically, to erythrocyte-derived depressing factor (Erythrocyte-Derived Depressing Factor, EDDF) extracted from human erythrocytes and its preparation Application in drugs for the treatment of hypertension. Background technique [0002] Hypertension is the most common cardiovascular disease, and its prevalence rate has reached more than 11% in China. With the development of economy, the improvement of living conditions and the change of lifestyle, the average life expectancy of people is prolonged, and hypertension prevalence is still on the rise. The pathogenesis of hypertension is very complex and has not been elucidated so far. In the past, the role of mental factors and humoral factors in blood pressure regulation and the pathogenesis of hypertension was focused on. In recent years, studies on cardiovascular physiology, pathophysiology, p...

Claims

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Application Information

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IPC IPC(8): A61K35/18A61P9/12C07K14/435
Inventor 文允镒
Owner THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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