Microarray chip of oligonucleotide and its preparing method

A technology of oligonucleotides and microarray chips, which is applied in the field of preparation of low-cost oligonucleotide microarray chips, can solve the problems of limiting the popularization and application of oligonucleotide microarray chips and high preparation costs, etc. Achieve the effect of reducing preparation cost, avoiding high cost and high reliability

Inactive Publication Date: 2003-08-27
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such high preparation costs greatly limit the popularization and application of oligonucleotide microarray chips.

Method used

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  • Microarray chip of oligonucleotide and its preparing method
  • Microarray chip of oligonucleotide and its preparing method
  • Microarray chip of oligonucleotide and its preparing method

Examples

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Embodiment 1

[0020] Embodiment 1: with reference to attached figure 1 A method for preparing an oligonucleotide microarray chip. Specifically as follows: 1, synthesize a universal oligonucleotide 1 (such as amino or thiol, etc.) with a special group label; 2, synthesize an oligonucleotide probe 2 without any group modification, the probe The needle consists of two parts (universal oligonucleotide complementary region 3 and probe-specific region 4), and the sequence of the probe-specific region is designed according to our needs; 3. The universal oligonucleotide 1 is combined with different oligonucleotides Nucleotide probe 2 is mixed in a centrifuge tube (Eppendorf tube) in equal proportions; 4. Place the above-mentioned Eppendorf tube in boiling water for 2 minutes to 5 minutes, and then naturally cool to room temperature for renaturation. 5. Spot the renatured product on the pre-treated substrate 5 (such as aldehyde or mercapto-coated glass slide) by a spotting device to form an oligonu...

Embodiment 2

[0021] Embodiment 2: with reference to attached figure 2 Another method for preparing an oligonucleotide microarray chip. Specifically as follows: 1. Synthesize a general oligonucleotide sequence 1 on a substrate 5 (such as a glass sheet or other material) by in situ synthesis technology; 2. Synthesize an oligonucleotide without any group modification Probe 2, the probe includes two parts (universal oligonucleotide complementary sequence 3 and target gene complementary sequence 4), the target gene complementary sequence 4 is designed according to our needs; Spot 2 nucleotide probes on the above-mentioned substrate to form a microarray chip; 4. Place the spotted chip in a humid hybridization box, and anneal at 42°C to 62°C for 4 hours; 5. Use 0.1% dodecyl Sodium alkyl sulfate (SDS) was used to remove unbound oligonucleotide probe 2, and then thoroughly washed with double distilled water, and dried for use.

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Abstract

The invention refers to a kind of oligopolynucleotide micro-array chip and the manufacturing method. The oligopolynucleotide probe is fixed on the base piece through a universal oligopolynucleotide segment and forms micro-array chip. The universal oligopolynucleotide chain may be embellished by special group (such as amido), then compounds with unnembellished oligopolynucleotide probe in centrifugal tube and forms double-chain. Finally it is fixed on specially worked glass piece or other material (such as aldehyde group) through sample applicator and forms the micro-array chip. Likewise, the universal oligopolynucleotide can be synthesized onto glass piece or other material through in situ synthesis technology, then uses the sample applicator to spot the unembellished oligopolynucleotide probe onto the glass piece or other material.

Description

technical field [0001] The invention relates to a method for preparing a low-cost oligonucleotide microarray chip. Through a common oligonucleotide chain, the oligonucleotide probe without any modification is immobilized on a glass slide or other materials to form a microarray chip. On the basis of retaining the high throughput, convenience, applicability and high reliability of the original chip, the technology greatly reduces the cost. technical background [0002] Gene chip is a high-tech developed rapidly in the field of life science in recent years. The so-called gene chip is a silicon chip, a glass slide, or a plastic chip on which a large number of gene probes or gene fragments are fixed in a specific arrangement. Gene chip technology is the main means to efficiently obtain relevant biological information on a large scale. At present, the application fields of this technology mainly include gene expression profile analysis, new gene discovery, gene mutation and pol...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 侯鹏陆祖宏祭美菊何农跃李松
Owner SOUTHEAST UNIV
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