Method for inhibiting algal growth using ultrasonic wave
An ultrasonic and algae technology, applied in biochemical equipment and methods, using electricity/wave energy to treat microorganisms, unicellular algae, etc.
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Embodiment 1
[0021] Table 1
[0022] It can be seen from Table 1 that the utilization frequency is 1.5-2.0MHz and the intensity is 0.5-0.6w / cm 2 Ultrasonic treatment of cyanobacteria can inhibit the growth of cyanobacteria, when the frequency is 1.7MHz, the intensity is 0.6w / cm 2 It has a better inhibitory effect on the growth of cyanobacteria.
Embodiment 2
[0025] Table 2
[0026] From Table 2 and figure 1 It can be seen from the figure that the OD value of group E treated in the logarithmic phase is close to that of the control group, and the growth resumes on the second day after treatment, the growth rate is equal to that of the control group, and the growth curve has been rising. No effect was apparent at the end of the trial. In group C, which received treatment during the stasis period, the growth was significantly inhibited during the whole process. After the culture period ended, its growth was only 47% of that of the control group, indicating that the physiological activities of cyanobacteria cells in the stagnant phase were still recovering, and their ability to resist external interference was weak. Ultrasonic treatment could make them receive more serious injury. The cyanobacteria cells in the logarithmic phase have vigorous metabolism, strong vitality, strong resistance ...
Embodiment 3
[0028] Treat each of the five groups of cyanobacteria culture fluids in the stagnation phase once, the frequency of the treatment is 1.7MHz, and the intensity is 0.6w / cm 2 , the treatment time of each group is different, respectively 1min, 3min, 5min, 7min, 9min, track the OD value of the whole culture period, draw its growth curve (OD value) control diagram, as figure 2 shown.
[0029] It can be seen from the figure that the growth of the group whose input time is 1 min is not affected, and maintains normal growth, and the growth rate and final biomass are the same as those of the control group; the growth of the group whose input time is 3 min is affected to a certain extent, and there is a short-term slow growth (1 to 2) days, it can still recover to a certain threshold quickly, and then grow at a normal growth rate, and the final biomass can reach more than 70% of the control group, and the inhibition effect is not ideal; the input time is 5min, 7min The three groups of ...
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