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Clavibacter and method for fermentation production of L-lysine

A technology of lysine and corynebacterium, applied in fermentation, bacteria, etc., can solve the problem of high production cost

Inactive Publication Date: 2003-05-07
上海新立工业微生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The second technical problem to be solved in the present invention is to disclose the method for producing L-lysine by fermenting the above strains, so as to overcome the high production cost defect of the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Fill 30ml of culture medium in a 500ml Erlenmeyer flask, which contains 175g / L glucose, 40g / L ammonium sulfate, 10ml / L cystine mother liquor (hair hydrolyzate), 5g / L corn steep liquor, 1g / L dihydrogen phosphate Potassium, 1g / L dipotassium hydrogen phosphate, 0.5g / L magnesium sulfate, 0.05g / L urea, 50g / L calcium carbonate (dissolved), pH 7.

[0090] The culture was sterilized at 120°C for 15 minutes. Take a ring of the parental strain and SNGBL 8 bacteria that have been pre-cultured for 24 hours from the slant of medium A, respectively, and insert them into the above-mentioned medium, and culture them with shaking at 32°C for 68 hours. The results are shown in the table below. Strains used Characteristics Yield (g / L) * Sugar conversion rate (%) 2305 wild type 1.4 0.8 A111 HS - 、AEC r 58.1 33.2 SNGBL8 HS - 、AEC r 、MF r , 82.6 47.2

[0091] TA r

[0092] * The amount of L-lysine is calculated as lysine hydrochloride

Embodiment 2

[0094] Corn starch is hydrolyzed by double enzymes to make about 25% hydrolyzed sugar solution. Partially hydrolyzed sugar solution is concentrated into 55% sugar solution by vacuum thin film concentration device.

[0095] In two Erlenmeyer flasks with a volume of 5L, respectively fill 0.8L medium containing 30g / L corn starch hydrolyzed sugar liquid, 10g / L ammonium sulfate, 3g / L urea, 1g / L dihydrogen phosphate Potassium, 1g / L dipotassium hydrogen phosphate, 0.4g / L magnesium sulfate, 0.3g / L (based on nitrogen content) soybean meal hydrolyzate, 0.1mg / L thiamine hydrochloride, 0.03mg / L biotin. The culture was sterilized at 120°C for 15 minutes. A ring of the parental strain and SNGBL8 bacteria that had been pre-cultured for 24 hours was taken from the slant of medium A and inserted into the above-mentioned medium, and then cultured at 32°C for 14 hours. The above steps are for seed culture.

[0096] In two small fermentation tanks with a volume of 16L, each tank is filled with...

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Abstract

The present invention discloses a coryneform CGMCC NO.0785 and a process for preparing L-lysine by its fermentation. Said process includes such steps as culturing coryneform CGMCC NO.0785, fermenting in culture medium, and collecting L-lysine from its fermented liquid. Its advantages are high output rate, high conversion rate of saccharide and low cost.

Description

technical field [0001] The invention relates to a Corynebacterium species and a production method of L-lysine, in particular to a production method through microbial fermentation. Background technique [0002] L-Lysine is a very important amino acid, which is mainly used as feed additive, food additive and pharmaceutical industry raw material. It is generally produced by microbial fermentation. [0003] At present, many patents and documents have disclosed the method of producing L-lysine by microbial fermentation, such as the technology disclosed in the Japanese Patent Application No. 55213 / 1967 bulletin, which utilizes a kind of S-(2-amino Ethyl)-L-cysteine ​​(called AEC) resistant bacterium; As Japanese patent application No. 36888 / 1974 announcement utilizes a kind of AEC resistance and L-leucine, L-homoserine auxotroph type of variants; such as the Japanese patent application No. 19235 / 1981 announcement utilizes a gamma-methyllysine resistant variant. [0004] There i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P13/08
Inventor 富英华沈永喜劳含章
Owner 上海新立工业微生物科技有限公司
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