Transgenic plants with increased seed yield, biomass and harvest index

A technology for monocotyledonous plants and plants, which is applied in the field of improving plant yield and can solve problems such as lack of grains

Inactive Publication Date: 2003-03-26
RES & DEV INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, shorter plants may also produce fewer grains

Method used

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  • Transgenic plants with increased seed yield, biomass and harvest index
  • Transgenic plants with increased seed yield, biomass and harvest index

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Embodiment 1

[0185] EXAMPLES Example 1: Genetic Analysis of Transgenic Wheat Plants

[0186] The initial pool of wheat transformants produced many independent transformants transgenic for Sh2-Rev6-HS and / or basta resistance.

[0187] Under controlled conditions in the greenhouse, T 0 Plants set seeds and mature.

[0188] Selected wheat transformants were analyzed by PCR for the presence of the introduced transgene and T 1 Segregation data for basta resistance of seeds.

[0189] PCR Screening of Transgenic Wheat Plants Genomic DNA samples prepared from leaf tissue were screened for the presence of Sh2-Rev6-HS using MC4Sh2 and MC35PUC19 (primer sequences given above) using a standard PCR protocol.

[0190] 27 independent transgenic wheat lines were tested. All 27 transgenic lines tested positive for basta resistance. Of the 27 transgenic lines tested for the presence of the Sh2-Rev6-HS transgene, 15 were positive and the other 12 were not. Example 2: Phenotypic ...

Embodiment 2

[0190] 27 independent transgenic wheat lines were tested. All 27 transgenic lines tested positive for basta resistance. Of the 27 transgenic lines tested for the presence of the Sh2-Rev6-HS transgene, 15 were positive and the other 12 were not. Example 2: Phenotypic Analysis of Transgenic Wheat Plants

[0191]Various phenotypic traits were collected and analyzed from 27 transgenic wheat plants grown in the greenhouse. As previously described, all 27 transgenic lines carried the herbicide resistance gene. These traits include: number of seeds per plant (seed / plant); individual seed weight expressed in milligrams per kernel (mg / kernel); harvest index (harvest index); The total weight of seeds expressed in (g / plant) (total seed weight); the number of grain heads per plant (heads); the total weight of plants expressed in grams per plant (g / plant) (plant weight); and The flag leaf weight (flag leaf weight) expressed in g / plant.

[0192] The seeds are uniformly dried to a moist...

Embodiment 3

[0206] Embodiment 3: rice experiment

[0207] Transgenic rice plants were generated as described in Materials and Methods. The resulting rice plants were analyzed as described in Examples 1 and 2.

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Abstract

The present invention provides methods for producing seeds and plants with increased biomass production. More specifically, the present invention provides methods for producing plants having increased yield for a number of plant traits, including seed number, seed weight, number of seed heads, flag leaf weight, and total plant weight. The present invention also provides methods for improving the harvest index of a plant. In a preferred embodiment, the method of the present invention comprises introducing into a plant a nucleic acid selected from the group consisting of a nucleic acid comprising SEQ ID NO: 3, which hybridizes to SEQ ID NO: 3 under high stringency conditions and encodes and retains SH2 - Nucleic acid of a polypeptide with biological activity of REV6-HS, fragment of SEQ ID NO: 3 encoding a peptide retaining biological activity of SH2-REV6-HS, encoding a polypeptide comprising SEQ ID NO: 4 or its SH2-REV6-SH organism Nucleic acids of fragments of biological activity, and nucleic acids encoding SH2HS or SH2RTS polypeptides. The present invention also relates to plants obtained by the methods provided herein.

Description

field of invention [0001] The present invention relates to improving plant yield, including both plant seed yield and plant biomass yield. More specifically, the present invention relates to transgenic plants having increased seed yield and biomass production relative to non-transgenic plants of the same genetic background. Even more particularly, the present invention relates to sh2-Rev6-HS transgenic plants and methods of producing these plants. Background of the invention [0002] ADP-glucose pyrophosphorylase (AGP) is one of the main enzymes involved in starch and glycogen biosynthesis in organisms such as plants, algae, fungi, and bacteria, especially plants. AGP catalyzes the following reactions: [0003] α-glucose-1-P+ATP→ADP-glucose+PPADP-glucose, the product of the above reaction, is the main glucose donor for starch biosynthesis in plants and glycogen biosynthesis in bacteria. [0004] AGP is widely distributed throughout the plant kingdom. ...

Claims

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Application Information

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IPC IPC(8): A01H5/00C12N9/12C12N15/09C12N15/82
CPCC12N15/8245C12N15/8261C12N9/1241Y02A40/146A01H1/00
Inventor M·吉罗克斯
Owner RES & DEV INST
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