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Immobile polyphenol oxidase catalyzed high-purity tea polyphenol process of producing high-purity theaflavin

A technology of polyphenol oxidase and immobilized enzyme, which is applied in the direction of fermentation, etc., can solve the problems of environmental pollution, unclear enzyme, unimmobilized enzyme, etc., and achieve the effect of reducing production cost, simple process steps, and less equipment investment

Inactive Publication Date: 2003-03-19
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the enzyme is a complex of various enzymes, the main active enzyme is not clear, and the enzyme is not immobilized, and the raw material for production is mainly the oxidation of fresh leaf phenols, and the theaflavin in the obtained product is 8-53%. Others are mainly oxidized thearubigin and theabrownin. In addition, acid and alkali need to be used repeatedly in the purification process of theaflavin, causing certain environmental pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Take the purified polyphenol oxidase enzyme solution and embed it in chitosan, cross-link it with glutaraldehyde, measure its activity 50U / mg, take a certain amount of immobilized polyphenol oxidase and 10 times of 6% tea polyphenol The solutions were mixed, stirred and reacted at 50°C for 60 minutes, and the obtained theaflavin solution was recovered by centrifugation to recover the immobilized enzyme, the theaflavin solution was extracted with ethanol, and the extraction ratio was theaflavin solution:purifying agent=1:8, Collect the purifying agent, concentrate the purifying agent into a paste, place the paste in a vacuum drying oven to dry at 55°C and a vacuum greater than 0.05Mpa, and dry it to a powder state to obtain a theaflavin content of 78.3%.

Embodiment 2

[0017] Take the purified polyphenol oxidase enzyme solution and embed it in alginate material and cross-link it with anhydrous butenedioic acid-ethylene to form it. The activity is determined to be about 35U / mg. Take a certain amount of immobilized polyphenol oxidase and 8 6% tea polyphenol solution was mixed, stirred and reacted at 50°C for 80 minutes, the obtained theaflavin solution was recovered by centrifugation for immobilized enzyme, the theaflavin solution was extracted with ethanol, and the extraction ratio was the theaflavin solution : Purifying agent=1: 8, collect purifying agent, purifying agent is concentrated to paste, paste is placed in vacuum oven for drying, condition is 50 ℃, and vacuum degree is greater than 0.05Mpa, is dried to powder state, and the tea yellow that obtains The element content is 83.1%.

Embodiment 3

[0019] Take the purified polyphenol oxidase enzyme solution and embed it in the alginate material and cross-link it with glutaraldehyde to form it. The activity is measured at 45U / mg. Take a certain amount of immobilized polyphenol oxidase and 10 times of 3.5% tea The phenol solutions were mixed, stirred and reacted at 50°C for 45 minutes, and the obtained theaflavin solution was centrifuged to recover the immobilized enzyme, and the theaflavin solution was extracted with ethanol, and the extraction ratio was theaflavin solution:purifying agent=1:8 , collecting the purifying agent, concentrating the purifying agent into a paste, placing the paste in a vacuum drying oven to dry at 55°C and a vacuum greater than 0.05Mpa, and drying to a powder state to obtain a theaflavin content of 81.3%.

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Abstract

The immobile polyphenol oxidase catalyzed high-purity tea polyphenol process of producing high-purity theaflavin includes fixing the polyphenol oxidase with embedding material, oxidizing and polymerizing high-purity tea polyphenol with the immobile polyphenol oxidase to produce high-purity tea polyphenol with the immobile polyphenol oxidase to produce high-purity theaflavin, solvent extracting theaflavin from its solution, and concentration and drying to obtain theaflavin product with 75% over purity. The technology process of the present invention is simple and has less equipment investment and low production cost. The fixation of the polyphenol oxidase makes it possible to perform enzymetic reaction continuously.

Description

technical field [0001] The invention relates to a method for producing theaflavins by processing tea extracts. Background technique [0002] In the existing high-activity theaflavin and its production technology, "high-activity theaflavin and its production method" was retrieved, and the patent application number is 00112536. It uses the enzyme system of fresh tea itself, under better oxidative polymerization conditions, Oxidize and polymerize the tea polyphenols in the added tea extract—tea polyphenols or the slag juice squeezed out of fresh tea leaves themselves into tea pigments mainly composed of theaflavins and thearubigins. Among them, the enzyme is a complex of various enzymes, the main active enzyme is not clear, and the enzyme is not immobilized, and the raw material for production is mainly the oxidation of fresh leaf phenols, and the theaflavin in the obtained product is 8-53%. Others are mainly oxidized thearubigin and theabrownin. In a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/22C12P17/06
Inventor 屠幼英夏会龙
Owner ZHEJIANG UNIV
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