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Process for extracting compound cordycepin and its Chinese medicine

An extraction method and a technology of cordycepin, which are applied in the field of compound cordycepin extraction and compound cordycepin traditional Chinese medicine preparations, can solve the problems of high toxicity, high price, and inability to be used in clinical medicine of cordycepin, and achieve small toxic and side effects and significant curative effect , High clinical use effect

Inactive Publication Date: 2003-02-12
吉林省吉庵堂医药研究所
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  • Description
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AI Technical Summary

Problems solved by technology

[0004] The invention discloses a method for extracting compound cordycepin and a traditional Chinese medicine preparation, which solves the problem that the existing cordycepin has high toxicity, high price and cannot be used in clinical medicine

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0016] The Cordyceps fungus is inoculated on a solid medium under a sterile strip, and when the fruiting body grows, it is taken out, dried and pulverized to obtain the Cordyceps fungus powder. Get the Cordyceps sinensis fungus powder and soak it in water for 12 hours, extract it with alcohol for 4 hours, filter to get the medicinal solution, concentrate and reclaim the ethanol to 300ml, put it on the ion column and remove impurities by eluting with alkaline solution (0.5N ammonia water), collect the solution and concentrate it into 30% of the original volume, refrigerated at 0°C for 24 hours, precipitated crystals, and recrystallized with n-butanol to obtain compound cordycepin.

Embodiment 2

[0018] Inoculate the Cordyceps sinensis strain in the liquid medium under aseptic conditions, the medium is: glucose 40g, peptone 20g, fresh milk 50ml, beef juice 20ml, KH 2 PO 4 1.0g, MgSO 4 0.5g, VB 1 10mg and 1000ml of distilled water are prepared. The culture room is cultivated for about 60 days. After the fruiting body grows, 20 liters of the fruiting body medium is removed and concentrated to 500ml. The ion column is used to elute the impurities, and the lotion is collected for refilling. Concentrate to 100ml, refrigerate at 0°C for 24 hours, precipitate crystals, and recrystallize with n-butanol to obtain compound cordycepin.

Embodiment 1

[0020] Powder injection: compound cordycepin 5mg

[0021] Sodium chloride 2.5g

[0022] Water for injection 250ml

[0023] Preparation process: Dissolve compound cordycepin and sodium chloride in an appropriate amount of distilled water for injection, adjust the pH to 8.5 with 1-5% sodium hydroxide solution, add water for injection to 1000ml, and filter through a 0.2μm microporous membrane The resulting solution was filled into ampoules under aseptic conditions and freeze-dried.

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Abstract

A process for extracting complex cordycepin includes such steps as immersing the powdered cordyceps in water for 8-14 hr, extracting by alcohol or by decocting and alcohol deposition, filtering to obtain liquid medicine, concentrating reflux of alcohol, eluting with ionic column, concentrating, cold storage at 0 deg.C for 24 hr, crystallizing, and recrystallizing with n-butanol. The cordycepin contains 2'-deoxyadensine and 3'-deoxyadenosine compound and has high anticancer action.

Description

Technical field: [0001] The invention discloses a method for extracting compound cordycepin, and at the same time provides a traditional Chinese medicine preparation of compound cordycepin, which belongs to the technical field of traditional Chinese medicine. Background technique: [0002] Cordyceps sinensis and Cordyceps militaris, as precious traditional Chinese medicines in my country, can be widely used in the technical field of traditional Chinese medicine. The main active ingredients contained in them are polypeptides, proteins, polysaccharides, cordycepin and nucleoside derivatives, which have antiviral, antibacterial, Anti-tumor, inhibit the growth of pathogens. At present, Sigma Company of the United States has successfully extracted cordycepin through chemical synthesis, which can only be used as a standard product of chemical reagents. In clinical medicine, it is very toxic and expensive, so it cannot be used as medicine, which affects the use of cordycepin. Medic...

Claims

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Application Information

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IPC IPC(8): A61K31/7076A61P35/00C07H19/16
Inventor 朱宏图肖红邹忆
Owner 吉林省吉庵堂医药研究所
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