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Dioscin and extraction method of diosgenin

A technology of diosgenin and an extraction method is applied in the field of extracting saponins, which can solve problems such as increasing production cost and environmental pollution, and achieve the effects of saving production cost, avoiding environmental pollution and reducing energy consumption.

Inactive Publication Date: 2003-02-05
张万举 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The bacillus stearothermophilus used in this method only serves as the fat-dissolving effect of solvent gasoline, and in addition, flammable and explosive ethanol and methanol have to be used for extraction, and the hydrolysis step still uses a large amount of hydrochloric acid as a catalyst, which increases the production cost environmental pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] In this example, fresh Dioscorea basilicum (also known as turmeric) is used as a raw material to extract dioscin according to the method of the present invention.

[0024]Use 100g of fresh two-year-old yam yam as raw material. Add water and pulverize, add lipase AS 2.1405 in the amount of 6μ / g to the pulverized raw material, ferment for 30 hours under stirring at 40°C, and maintain pH 7 during the fermentation process, and initially separate impurities such as cellulose and lignin in the form of filter residue come out. After fermentation, let it stand for 10 hours to precipitate the starch, recover the supernatant, suspension and starch respectively, first mix the suspension and filter residue to make the solid-liquid ratio 1:3 to 1:4, boil for 1 hour, and carry out Filter, repeat like this twice, and take back the filter residue for later use. Combine three filtrates, concentrate under reduced pressure to 10 ° Be, add ethanol, make the ethanol content in the solutio...

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PUM

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Abstract

The method for extracting dioscin and diosgenin includes the following steps: pulverizing rhizome of Chinese yam plant by adding water, fermentation by using lipolytic enzyme, separating cellulose and lignin, recovering supernatant, suspension and starch, mixing recovered liquors and filtering residue, boiling and filtering, repeating for several times, filtering residue for stand-by, combining filtrates and concentrating, using ethyl alcohol to make reflux extraction, cooling and filtering, decompressing and concentrating filtrate, adsorbing, evaporating, ethyl alcohol extracting, adding ethyl ether, cooling to room temp. and separating out tetrasaccharide saponin, recovering ethyl alcohol and ethyl ether from supernatant, precipitating out saponin, drying, adding water in starch and filter residue, regulating pH, adding alpha-amylase.

Description

[0001] Technical Field The present invention relates to a method for extracting saponins from Dioscoreaceae plants. Specifically, the present invention relates to a method for extracting saponins and sapogenins from Dioscoreaceae plants by using biotechnology. Background technique [0002] Plants of the Dioscoreaceae family include Dioscorea scutellariae (also known as turmeric), and Dioscorea mongolica (also known as Dianosaurus), etc., which are rich in steroidal saponins and are important raw materials for the synthesis of steroid hormone drugs. [0003] In the prior art, there are many methods for extracting saponins from diosgenaceous plants. For example, CN1008938 records a kind of dioscin-containing diosgenaceous plants as raw material, using Aspergillus niger for fermentation, which can jointly produce dioscin and citric acid. Item product. Through fermentation, the molecular structure of dioscin remains unchanged, and the yield of dioscin is doubled; after fermentatio...

Claims

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Application Information

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IPC IPC(8): C07J71/00
Inventor 张万举周康康王福祥
Owner 张万举
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