Method of in vitro culturing medicine ox gallstone with natural ox gall

A technology for ox gallstones and ox bile is applied in the directions of pharmaceutical formulations, medical preparations containing active ingredients, unknown raw materials, etc., which can solve the problems that ox gallstones cannot meet medicinal requirements, and achieve good social and economic benefits. The effect of low production cost and high stone formation efficiency

Inactive Publication Date: 2002-05-08
武汉键民大鹏药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the bovine gallstones made by the above method cannot meet the medicinal requirements of the new "Chinese Pharmacopoeia" for the bovine gallstones.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0022] (1) Acquisition of natural ox bile: take out fresh ox bile, adjust the taurine content to 7% according to the requirements of high-quality bezoar, put it in a sterilized tank, and store it at low temperature for later use.

[0023] (2) Preparation of fermented ox bile: place ox bile in a fermenter, inoculate the combined bacterial solution of Escherichia coli and Proteus into the ox bile according to the ratio of inoculating 100 ml of bacterial cell solution per 10000 ml of ox bile, and ferment for 80 hours for later use .

[0024] (3) Preparation of compound calcium bilirubin:

[0025] ①Take 20,000ml of fermented ox bile and add 22,000ml of clarified saturated calcium oxide solution, stir, boil, and after cooling, take out the brown-red precipitate.

[0026] ② Add 10,000ml of purified water to the brown-red precipitate, stir thoroughly, pour off the supernatant after standing still, add bilirubin until its content reaches 45%, then add 3g of zinc sulfate and 3g of mag...

Embodiment approach 2

[0032] (1) Acquisition of natural ox bile: when the cattle are slaughtered, the gallbladder is removed under aseptic conditions, and the gallbladder is selected to be free from impurities, uncontaminated, and golden in color, and the bile is poured into a sterilized tank. According to the requirements of high-quality bezoar, Adjust the taurine to make its content to 6%, and store it at low temperature for future use.

[0033] (2) Preparation of fermented ox bile: place ox bile in a fermenter, inoculate Escherichia coli into the ox bile at a ratio of inoculating 200 ml of bacterial cell solution per 10000 ml of ox bile, ferment for 96 hours, and set aside.

[0034] (3) Preparation of compound calcium bilirubin:

[0035] ①Take 20,000ml of fermented ox bile and add 60,000ml of clarified saturated calcium hydroxide solution, stir, boil, cool to 50°C, and take out the brown-red precipitate.

[0036] ②Add 10,000ml of cold boiled water to the brown-red precipitate, stir and let it s...

Embodiment approach 3

[0042] (1) Acquisition of natural ox bile: take out fresh ox bile, put it into a sterilized tank, and store it at low temperature for later use.

[0043] (2) Preparation of fermented ox bile: place the ox bile in a fermenter, inoculate 10000 ml of ox bile with 300 ml of bacterial cell solution, inoculate Enterococcus into the ox bile, ferment for 92 hours, and set aside.

[0044] (3) Preparation of compound calcium bilirubin:

[0045] ① Take 29600ml of fermented ox bile, filter and add 88800ml of clarified saturated calcium oxide solution, stir, boil, and take out the brown-red precipitate after cooling.

[0046] ② Add water to the brownish-red precipitate and stir thoroughly, pour off the supernatant after standing still, then add bilirubin until its content reaches more than 45%, then add 100g of cholic acid, 40g of deoxycholic acid, 3g of zinc sulfate, and 3g of magnesium sulfate, Stir and mix well.

[0047] (4) Cultivate medicinal bovine gallstones at room temperature of 1...

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Abstract

Natural ox gall is first taurine regulated and bacteria fermented to prepare fermented ox gall. The fermented ox gall is gall is then mixed with clear saturated calcium salt solution through stirring, heated to boil, filtered to obtain red brown precipitate, which is stirred, washed, filtered to eliminate supernatant and mixed with bilirubin, cholalic acid, deoxycholic acid, inorganic salt and other effective components to obtain composite bilirubin calcium. Fermented ox gall and bilirubin calcium in certain proportion are mixed and the pH of mixture is regulated to below 6.8 and directional off axis rotated to form gallstone, and the gallstone is rest cultured, taken out and dried. The said process can obtain ox gallstone product with the same characteristics, structure, component and medicinal effect as high quality natural bezoar.

Description

1. Technical field [0001] The invention belongs to the technical field of pharmacy, and in particular relates to a method for manufacturing a traditional Chinese medicine raw material. 2. Background technology [0002] Bovine gallstone, namely bezoar, is a precious Chinese herbal medicine. It has the functions of clearing heart, clearing phlegm, resuscitating, cooling liver, relieving wind, clearing heat and detoxifying. Many famous and precious Chinese patent medicines in our country, such as Angong Niuhuang Pills and Liushen Pills, are all formulated with bezoar as the main medicine. However, the natural stone formation rate in cattle is only 1-2%. Therefore, the source of medicine is extremely scarce, expensive, and dependent on imports, which not only costs a lot of foreign exchange, but also restricts the development of my country's traditional medicine industry. [0003] Since 1956, Chinese medical workers have been actively engaged in the research of artificial bezo...

Claims

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Application Information

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IPC IPC(8): A61K35/413
Inventor 蔡红娇
Owner 武汉键民大鹏药业有限公司
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