Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Process for preparing optical pure (S)-2-octanol by microorganism and its special microorganism

A microbiological and optical technology, applied in the field of biological separation of racemic compounds

Inactive Publication Date: 2006-11-01
JIANGNAN UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] Chinese patent CN1366550A has reported a kind of (R)-2-octanol dehydrogenase, which can preferentially oxidize (R)-2-octanol in the two optical isomers of 2-octanol, but it cannot Racemic 2-octanol is converted as a substrate to obtain (R)-2-octanol, and in the application of chiral alcohol preparation, only ketone is used as a substrate to reduce the corresponding chiral alcohol, rather than racemate as Substrates undergo a process of chiral resolution to yield optically pure products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Process for preparing optical pure (S)-2-octanol by microorganism and its special microorganism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Cultivation of bacterial strains: The composition of the medium is that the components contained in each 100ml of culture solution are in grams: glucose 0.25-1.0, meat extract 0.5-2.0, peptone 0.5-2.0, yeast extract 0.1-0.5, (NH 4 ) 2 HPO 4 0.5~1.0,KH 2 PO 4 0.25~0.5, MgSO 4 ·7H 2 O 0.025~0.1, NaCl 0.001~0.005, ZnSO 4 ·7H 2 O 0.001~0.005, FeSO 4 ·7H 2 O 0.001~0.005, CuSO 4 ·5H 2 O 0.0001~0.0005, MnSO 4 4H 2 O0.0001~0.0005.

[0058] The culture conditions are as follows: the initial pH is 6.0-8.0, the filling volume is 30%, the culture temperature is 25° C., the rotation speed of the shaking flask is 100-300 rpm, and the culture time is 72 hours.

Embodiment 2

[0060] Cultivation of the bacterial strain: the composition of the culture medium is the same as in Example 1. The culture conditions are as follows: the initial pH is 6.0-8.0, the filling volume is 5%, the culture temperature is 35° C., the rotation speed of the shaking flask is 100-300 rpm, and the culture time is 24 hours.

Embodiment 3

[0062] Preparation of whole cells: Take a loop of P.maltophilia CCTCC M204042 from the slope and inoculate it in a 250ml shaker flask with 30% culture solution at 25°C and 100-300rpm for 72 hours; The body was centrifuged and washed twice with saline, and the whole cells of the strain were collected for transformation reaction.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
optical purityaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for preparing optically pure (S)-2-octanol by a microbial method and a special microorganism thereof, which belong to the technical field of separating racemic compounds by a biological method. The present invention screens and obtains Pseudomonas maltophilia SYB-4 CCTCC M204042. Utilize this bacterial strain after cultivating, the preparation of whole cell, carry out the catalytic conversion reaction of microbial cell with racemic 2-octanol as substrate, asymmetric conversion racemic 2-octanol obtains product (S)-2-octanol, The optical purity of the product reaches above 80% e.e. The present invention determines the reaction system and reaction conditions for the asymmetric transformation of racemic 2-octanol in the whole cell of the strain, and the optical purity of the product is improved; the present invention also helps to understand the biological diversity of racemic compound resolution , which is of great significance for the development of special enzyme sources for resolution and the research of resolution methods in the future.

Description

technical field [0001] The invention discloses a method for preparing optically pure (S)-2-octanol by a microbial method and a special microorganism thereof, which belong to the technical field of separating racemic compounds by a biological method. Background technique [0002] The chemical structure of 2-octanol is: [0003] [0004] Racemic 2-octanol consists of two enantiomers (R)-2-octanol and (S)-2-octanol. [0005] Optically pure 2-octanol, including (R)-2-octanol and (S)-2-octanol, is not only an anabolic steroid, vitamin E, and insect pheromones (bioinsecticides) but also many optically active drugs and It is an important chiral intermediate of pesticides, and is an indispensable and important chiral raw material for the preparation of high-performance liquid crystals and their devices. Its optical activity and high dielectric constant are closely related to many properties of liquid crystals; as an important intermediate, it is also Used in organic synthesis o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/04C12R1/38
Inventor 徐岩聂尧穆晓清
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com