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Short lactobacillus producing gamma-aminobutyric acid and use thereof

A kind of Lactobacillus brevis, aminobutyric acid technology, applied in the direction of microorganism-based methods, bacteria, biochemical equipment and methods, etc., can solve problems such as safety and health risks

Inactive Publication Date: 2006-09-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, Escherichia coli was usually used as the strain for the biosynthesis of GABA, but the use of Escherichia coli as the production strain has hidden dangers in safety and sanitation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 3

[0015] After the strain was activated on the agar slant medium, it was transferred to the MRS seed medium, and the culture time was 20 hours. The seed culture solution was inoculated in the GYP fermentation medium with an inoculum of 1%, and 1% of a Sodium L-glutamate hydrate, the volume of the fermentation medium is 200mL / 500mL, and it is cultured statically for 60 hours to obtain the γ-aminobutyric acid fermentation liquid, which is analyzed by high performance liquid chromatography, and the content is about 2g / L.

Embodiment 4

[0017] After the strain was activated on the agar slant medium, it was transferred to the MRS seed medium, and the culture time was 36 hours. The seed culture solution was inoculated in the MRS fermentation medium with an inoculum of 1%, and 1% of a Sodium L-glutamate hydrate, the volume of the fermentation medium is 200mL / 500mL, and it is cultured statically for 60 hours to obtain the γ-aminobutyric acid fermentation broth, which is analyzed by high performance liquid chromatography, and the content is about 2g / L.

Embodiment 5

[0019] After the strain was activated on the agar slant medium, it was transferred to the GYP seed medium for 36 hours. The seed culture solution was inoculated in the GYP fermentation medium with 2% inoculation amount, and 1% of a Hydrated L-sodium glutamate (L-MSG), the liquid content of the fermentation medium is 200mL / 500mL, and it is cultured statically for 60 hours to obtain the γ-aminobutyric acid fermentation liquid, which is analyzed by high performance liquid chromatography. The content is about 4g / L.

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PUM

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Abstract

The present invention discloses one kind of gamma-aminobutyric acid producing lactobacillus brevis and its use. The lactobacillus brevis has preservatioin number of CGMCC No. 1306; exhibits pairs or chains; and has no motion, no spore, small colony and smooth surface. It belongs to Gram positive bacteria and facultative anaerobe, and may be used in producing gamma-aminobutyric acid. By means of biological synthesis process, the high activity glutamate decarboxylase connected in the lactobacillus brevis and sodium L-glutamate as substrate, the sodium L-glutamate is decarboxylated to produce gamma-aminobutyric acid and CO2. Thus produced gamma-aminobutyric acid is safe in food level.

Description

technical field [0001] The invention relates to a brevis lactobacillus producing gamma-aminobutyric acid and its application. Background technique [0002] So far, humans have discovered about dozens of neurotransmitters. Studies have proved that the abnormality of some amino acid transmitters is one of the factors that induce various neurological diseases (such as epilepsy and Parkinson's disease). γ-Aminobutyric acid (GABA for short), also known as aminobutyric acid, is an important non-protein amino acid. As an important neurotransmitter in the central nervous system, it has important physiological functions. A large number of literatures have reported that GABA can lower blood pressure and cholesterol, prevent overheating of nerve cells, so it has calming and antidepressant effects, and can also enhance brain function and long-term memory, increase growth hormone secretion, prevent obesity, strengthen liver and kidney, and improve menopause Syndrome and other functions...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/24C12P13/00C07C229/08C12N
Inventor 梅乐和黄俊夏江
Owner ZHEJIANG UNIV
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