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De-ester Yarrowia liplytica CGMCC No.0692 and its use in petroleum refinement

A yeast strain and petroleum technology, which is applied in the petroleum industry, refined hydrocarbon oil, fungi, etc., can solve the problems of long production time per unit oil product, dewaxing, poor demulsification effect, short life activity, etc., and achieve easier Demulsification, shortening of dewaxing time, and strong life activity

Inactive Publication Date: 2005-07-06
PETROCHINA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the above-mentioned microbial petroleum refining process, the strain of Torulopsis dewaxing (selected by Karamay Oilfield) is generally selected. This petroleum yeast strain has short life activity, poor dewaxing and demulsification effects, and low production efficiency, making the unit Oil production time is long and energy consumption is high

Method used

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  • De-ester Yarrowia liplytica CGMCC No.0692 and its use in petroleum refinement
  • De-ester Yarrowia liplytica CGMCC No.0692 and its use in petroleum refinement
  • De-ester Yarrowia liplytica CGMCC No.0692 and its use in petroleum refinement

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: According to the attached drawings figure 1 Process operation, obtain 45# transformer oil base oil.

[0038] In a sterile room, transfer the yeast of the present invention to a small shake flask, a large shake flask, a first-stage tank, a third-stage tank, and five-stage culture and proliferation, and connect it to the fermentation tank containing raw oil. After 30 hours of fermentation, take a sample to measure the freezing point Qualified, stop tank, demulsification, sedimentation, centrifugation, low freezing point 45# transformer oil base oil and petroleum yeast waste liquid can be obtained.

Embodiment 2

[0040] According to the attached picture figure 2 Process operation and obtain 22# refrigerating machine oil base oil.

[0041] In the sterile room, transfer the yeast to the small shake flask, the large shake flask, the first-level tank, the third-level tank and the five-level culture and proliferation, and connect it to the fermentation tank containing raw oil. After 100 hours of fermentation, the sampling and pour point are qualified. , Stop tank, demulsify, settle, centrifuge, you can get low freezing point 22# refrigerating machine oil base oil and petroleum yeast waste liquid.

Embodiment 3

[0043] According to the attached picture image 3 Process operation and obtain 32# refrigerating machine oil base oil.

[0044] In the sterile room, transfer the yeast to the small shake flask, large shake flask, first-level tank, third-level tank and five-level culture and proliferation, and connect it to the fermentation tank containing raw oil. After 60 hours of fermentation, the sampling test pour point is qualified , Stop tank, demulsify, settle, centrifuge, you can get low freezing point 32# refrigerating machine oil base oil and petroleum yeast waste liquid.

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Abstract

The present invention relates to a strain of Yarrowia lipolytica and its application method in petroleum refining. The strain of Yarrowia lipolytica CGMCC NO.0692 is characterized by oval, oval, columnar to long column shape, the size is (2-5.5)×(3.5-15.5)μm 2 , formed by rings and veils; hyphae, produced by pseudohyphae and mycelium; color, colony cheese-like, milky white to off-white, surface wrinkled, reflective, and edges are root-like or wavy. The invention provides a method for refining petroleum strains. The yeast culture is first cultivated on an inclined plane at a temperature of 25-30° C., then moved to small and large shaker flasks for cultivation, and then sequentially enters the first-level and third-level seed tanks for fermentation for 10-18 hours respectively. It is ventilated, pH 3.5-4, and then enters the production tank together with raw material oil, water, and culture medium for mixed fermentation for 30-100 hours. After reaching the control index, it is heated and demulsified to make oil products. The invention has stronger life activity, high dewaxing efficiency, easy demulsification, high production efficiency, can improve the properties of the base oil, and obtain a low-condensation oil product with better performance.

Description

Technical field [0001] The invention relates to a technique for refining petroleum by using microbial fermentation, and is a Yarrowia lysate strain and its application method in petroleum refining. Background technique [0002] The use of microbial oil refining technology has been reported in 1906, mainly for the microbial oxidation of petroleum gas and methane, focusing on the oxidation mechanism of low-grade petroleum hydrocarbons and other basic research. By 1963, France reported the oxidation method of petroleum hydrocarbons, and other countries successively carried out petroleum fermentation research and obtained industrialization results. At present, microbial methods can be used to produce low-condensing lubricant products and base oils. Since microbial fermentation is generally carried out at room temperature and pressure, it avoids the high temperature and high pressure conditions required in the production of petrochemical products by conventional methods, greatly reduc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C10G32/00C12N1/16C12S1/00
Inventor 王建成黎鹏刘成林李娅娅孙翠云
Owner PETROCHINA CO LTD
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