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De-ester Yarrowia liplytica CGMCC No.0692 and its use in petroleum refinement

A yeast strain and petroleum technology, which is applied in the petroleum industry, refined hydrocarbon oil, fungi, etc., can solve the problems of dewaxing, poor demulsification effect, long production time per unit oil product, low production efficiency, etc., to achieve Easier demulsification, shorter dewaxing time, and lower unit energy consumption

Inactive Publication Date: 2004-07-07
PETROCHINA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the above-mentioned microbial petroleum refining process, the strain of Torulopsis dewaxing (selected by Karamay Oilfield) is generally selected. This petroleum yeast strain has short life activity, poor dewaxing and demulsification effects, and low production efficiency, making the unit Oil production time is long and energy consumption is high

Method used

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  • De-ester Yarrowia liplytica CGMCC No.0692 and its use in petroleum refinement
  • De-ester Yarrowia liplytica CGMCC No.0692 and its use in petroleum refinement
  • De-ester Yarrowia liplytica CGMCC No.0692 and its use in petroleum refinement

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Experimental program
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Effect test

Embodiment 1

[0038] Embodiment one: according to the attached drawing figure 1 Process operation to obtain 45# transformer oil base oil.

[0039] In a sterile room, transfer the yeast of the present invention to a small shake flask, a large shake flask, a first-stage tank, and a third-stage tank for five-stage culture and proliferation, and connect it to a fermenter containing raw material oil. After 30 hours of fermentation, take a sample to measure the freezing point Qualified, stop the tank, demulsify, settle, and centrifuge to obtain low freezing point 45# transformer oil base oil and petroleum yeast waste liquid.

Embodiment 2

[0041] According to the attached picture figure 2 Process flow operation to obtain 22# refrigerating machine oil base oil.

[0042] In the sterile room, the yeast was transferred to small shake flasks, large shake flasks, first-level tanks, and third-level tanks for five-level culture and multiplication, and then connected to the fermentation tank containing raw material oil. After 100 hours of fermentation, sampling and measuring pour point passed , stop the tank, demulsify, settle, and centrifuge to obtain low freezing point 22# refrigerating machine oil base oil and petroleum yeast waste liquid.

Embodiment 3

[0044] According to the attached picture image 3 Process flow operation to obtain 32# refrigerating machine oil base oil.

[0045] In the sterile room, the yeast was transferred to small shake flasks, large shake flasks, first-level tanks, and third-level tanks for five-level culture and proliferation, and then connected to a fermentation tank containing raw material oil. After 60 hours of fermentation, sampling and measuring pour point passed , stop the tank, break the emulsion, settle, and centrifuge to obtain low freezing point 32# refrigerating machine oil base oil and petroleum yeast waste liquid.

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Abstract

A Yarrowia lipolytica (GMCC No.0692) and its application in refining petroleum are disclosed. Said application process includes such steps as slant culturing at 25-30 deg.C, shake culturing, fermenting respectively and sequentially in stage-one and stage-three seeding tanks for 10-18 hr, mixing with raw oil, water and culture medium, fermenting in production tank for 30-100 hr, heating and emulsifying. Its advantages are high activity, dewaxing efficiency and productivity, easy demulsifying, and high quality of oil product.

Description

technical field [0001] The invention relates to the technique of refining petroleum by using microbial fermentation, and is a strain of Yarrowia esterolytica and its application method in petroleum refining. Background technique [0002] In 1906, the technology of microbial oil refining was reported, mainly for the microbial oxidation of petroleum gas and methane, and the oxidation mechanism of low-level petroleum hydrocarbons and other basic researches were carried out. By 1963, France reported the oxidation method of petroleum hydrocarbons, and then other countries successively carried out petroleum fermentation research and obtained industrialization results. At present, low-point lubricating oil products and base oil products can be produced by using microbial methods. Since microbial fermentation is generally carried out at normal temperature and pressure, the high temperature and high pressure conditions required for the production of petrochemical products by conventi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C10G32/00C12N1/16C12S1/00
Inventor 王建成黎鹏刘成林李娅娅孙翠云
Owner PETROCHINA CO LTD
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