Electrochemical biosensor and application thereof

Abstract

The invention provides an electrochemical biosensor, which is a novel electrochemical homogeneous biosensing platform based on steric hindrance of electrically induced electroreactive dyes and proteins, and is used for simultaneously detecting four parameter indexes in serum and performing typing detection. Porous UiO-66-NH2 is adopted as a nanometer container to load electroactive basic dyes, namely neutral red (NR), toluidine blue (TB), 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) and malachite green (MG) (NR-coated UiO-66, TB-coated UiO-66, TMB-coated UiO-66 and MG-coated UiO-66), and the surface acting force between the dyes and the UiO-66 nanometer material is Pi-Pi force. According to the application of the biosensor, the sensitivity reaches up to pg / mL, and the response time is 60 minutes. In addition, the immobilization-free electrochemical biosensing method also has good sensing performance when being used for detection in an actual sample. Compared with other sensing strategies, the electrochemical platform only needs one antibody probe, the reaction only needs one step, the time is shorter, and the operation steps are simple. The invention provides a rapid and sensitive method for breast cancer typing analysis.

Description

technical field [0001] The invention relates to an electrochemical biosensor and an application thereof, in particular to the construction of a homogeneous electrochemical biosensor based on electrically induced electroactive dyes and the steric hindrance effect of proteins, and its application in identifying estrogen receptor (ER) in serum , progesterone receptor (PR), human epidermal growth factor receptor-2 (HER-2) and cell proliferation biomarker (Ki-67) four biomarkers and methods applied to the detection of breast cancer in serum . Background technique [0002] In the prior art, estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor-2 (HER-2) and cell Proliferation biomarkers (Ki-67) All four biomarker methods have significant deficiencies. Either qualitative and semi-quantitative studies can only be performed on some key molecules. The relationship between key molecules and clinical information cannot be displayed in real time, i...

AI Technical Summary

Problems solved by technology

The relationship between key molecules and clinical information cannot be displayed in real time, it is difficult to accurately reveal the process of tumor progression, and it is difficult to comprehensively evaluate tumor invasion behavior in macroscopic form

More importantly, these techniques need to extract and process tumor cells or tissue samples from the body, losing the three-dimensional information of the primary tumor, and invasive detection methods may lead to tumor metastasis

[0003] Although researchers in this field have focused on detecting single or multiple biomarkers on the same system, there is currently no simultaneous detection of four biomarkers to analyze breast cancer molecular types by detection of HER2, ER, PR and Ki67 in serum electrochemical platform

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