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Application of asiatic acid in preparation of medicine for treating hepatitis B

A technology of hepatitis B and asiatic acid, which is applied in the field of biomedicine to achieve the effect of reducing the infection rate and having great application prospects

Pending Publication Date: 2022-07-29
重庆医科大学国际体外诊断研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Inhibition of HBx function may inhibit cccDNA transcription

Method used

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  • Application of asiatic acid in preparation of medicine for treating hepatitis B
  • Application of asiatic acid in preparation of medicine for treating hepatitis B
  • Application of asiatic acid in preparation of medicine for treating hepatitis B

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] 1. Experimental method

[0074] 1. Cell culture

[0075] HepG2-NTCP cells were cultured in DMEM medium containing 10% fetal bovine serum, 1% penicillin-streptomycin, 2ug / mL doxycycline, and HepG2.2.15 cells were cultured in 10% fetal bovine serum, 1% penicillin- Streptomycin, 400ug / mL G418 DMEM medium, placed in a 37 ℃, 5% CO2 incubator for culture. Asiatic acid (cas No. 464-92-6) was purchased from MCE.

[0076] 2. Plasmids and drugs

[0077] prcccDNA and pCMV-KRAB-Cre were gifted by Prof. Qiang Deng (Fudan University, Shanghai, China). 3×Flag-HBx was constructed by inserting full-length HBx into p3×Flag-CMV7.1. 3×Flag-HBx was constructed by inserting full-length HBx into p3×Flag-CMV7.1. 2×HA HBx was a gift from Prof. Chen Jieliang (Fudan University, Shanghai, China). Asiatic acid (cas No. 464-92-6) was purchased from MCE Company, dissolved in DMSO, prepared as a 20 mM stock solution, aliquoted and stored at –80°C for use.

[0078] 3. MTT test

[0079] HepG2-NT...

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Abstract

The invention belongs to the technical field of biological medicines, and particularly discloses application of asiatic acid to preparation of a medicine for treating hepatitis B. It is found that asiatic acid can reduce the HBx protein level and inhibit the transcriptional activity of cccDNA so as to reduce the total HBV RNAs and HBV 3.5-kb RNA levels in cells and the HBsAg level in supernatant, and can effectively reduce the HBsAg and HBV DNA levels in serum and the HBV RNA and HBV DNA levels in liver tissue in a recombinant cccDNA mouse model, and the asiatic acid is a potential novel HBx targeting medicine and can be used for treating hepatitis B. The invention provides a new direction and theoretical basis for reducing the infection rate of HBV and exploring a novel medicine for effectively treating hepatitis B.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the use of asiatic acid in the preparation of a hepatitis B treatment medicine. Background technique [0002] Hepatitis B virus (HBV) infection is a major public health problem worldwide. According to WHO reports, about 2 billion people in the world have been infected with HBV, of which there are about 257 million patients with chronic hepatitis B infection. my country is a high-endemic area of ​​HBV. There are about 97 million hepatitis B virus carriers, accounting for 8% to 10% of the total population of the country, of which about 20 million are chronic hepatitis B patients, and there are as many as 263,000 deaths due to complications such as liver cirrhosis and liver cancer caused by chronic hepatitis B every year. . Therefore, the task of hepatitis B prevention and treatment is very serious. [0003] Currently, the commonly used antiviral drugs for the treatment of c...

Claims

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Application Information

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IPC IPC(8): A61K31/56A61P31/20A61P1/16
CPCA61K31/56A61P31/20A61P1/16
Inventor 陈娟杨祯程胜桃
Owner 重庆医科大学国际体外诊断研究院
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