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Electrochemical method for high-selectivity detection of hydrogen sulfide in living body by using nano-silver carbon fiber microelectrode pretreated by sodium sulfide

A carbon fiber and microelectrode technology, applied in the field of analysis, can solve the problems of interference hydrogen sulfide existence form, difficult detection, interference, etc., and achieve the effect of avoiding oxidative stress reaction of the body

Pending Publication Date: 2022-07-26
RENMIN UNIVERSITY OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the complexity of the test samples, the interference of sulfur-containing compounds, and the different forms of hydrogen sulfide under different physiological conditions, it is difficult to detect accurately in biological samples.
[0003] The use of silver nanoparticles combined with hydrogen sulfide to form silver sulfide precipitation can cause potential changes to achieve the purpose of detecting hydrogen sulfide, but when this method is applied to the detection of biological samples, it is susceptible to interference from other sulfur-containing compounds and electroactive substances, so The method needs to be improved

Method used

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  • Electrochemical method for high-selectivity detection of hydrogen sulfide in living body by using nano-silver carbon fiber microelectrode pretreated by sodium sulfide
  • Electrochemical method for high-selectivity detection of hydrogen sulfide in living body by using nano-silver carbon fiber microelectrode pretreated by sodium sulfide
  • Electrochemical method for high-selectivity detection of hydrogen sulfide in living body by using nano-silver carbon fiber microelectrode pretreated by sodium sulfide

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preparation example Construction

[0050] In the first aspect of the present invention, the present invention proposes a method for pretreating carbon fiber microelectrodes with sodium sulfide. In vitro performance testing of this electrode was performed in advance prior to in vivo analysis. Preparation method reference figure 1 , the method includes:

[0051] S100: Ag nanoparticles deposited on the surface of carbon fiber microelectrodes

[0052] The carbon fiber microelectrodes were placed in a solution containing 1 mmol / L KNO 3 and 0.25mmol / L AgNO 3 In the mixed electrolyte of , silver nanoparticles were modified on the surface of the carbon fiber microelectrode by electrodeposition, and the electrodeposition used potentiostatic deposition, the condition was -0.2V, and the deposition time was 200s.

[0053] The above-mentioned electrodeposition may be carried out in a two-electrode system or a three-electrode system. In the two-electrode system, the working electrode is the carbon fiber microelectrode, ...

Embodiment 1

[0063] A carbon fiber microelectrode was used as the detection electrode.

[0064] Preparation of carbon fiber electrodes: First, a glass capillary (outer diameter: 1.5 mm; inner diameter: 0.89 mm; length: 10 cm) was drawn on a microelectrode drawing instrument (WD-1 type, Chengdu Instrument Factory) into two very thin tips For the conical capillary, under the microscope, lightly cut the tip with a glass knife so that the inner diameter of the port is about 30-50 μm. Then, about 2 cm long carbon fiber was glued to a copper wire with silver conductive glue, and it was threaded into the drawn capillary, so that the carbon fiber was exposed to a length of about 200-500 μm outside the tip of the capillary. Seal both ends of the capillary with insulating glue to fix the copper wire and the capillary together, and under the microscope, use a knife to cut the part of the carbon fiber protruding from the capillary to 200-500 μm to make a carbon fiber microelectrode.

[0065] The prep...

Embodiment 2

[0073] Nano-silver carbon fiber microelectrodes pretreated with sodium sulfide for highly selective detection of hydrogen sulfide in the living nervous system

[0074] To implant microelectrodes into the hippocampus of anesthetized rats, we injected S 2- 100 μM artificial cerebrospinal fluid, such as Figure 5 As shown by the blue star, a drop in potential can be observed after 300 s, injected with 200 μM Cu 2+ artificial cerebrospinal fluid ( Figure 5 Black star), the potential almost recovered to the starting baseline, proving that hydrogen sulfide can be detected by the electrode through the circulation of body fluids, and with Cu 2+ Hydrogen sulfide was consumed to aid our results. The above results indicate that the microelectrodes can track the changes of hydrogen sulfide in vivo and the physiological and pathological processes related to hydrogen sulfide in vivo.

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Abstract

The invention relates to an electrochemical method for high-selectivity detection of hydrogen sulfide in an organism by using a nano-silver carbon fiber microelectrode pretreated by sodium sulfide. The invention discloses an electrochemical method for high-selectivity detection of hydrogen sulfide in a cranial nervous system by using a nano-silver carbon fiber microelectrode. The method comprises the following steps: electro-depositing silver nanoparticles on the surface of a carbon fiber microelectrode by adopting an electro-deposition method, and pre-treating the electrode in a sodium sulfide solution to generate a layer of silver sulfide film on the surface of the electrode so as to regulate and control the surface of the microelectrode, thereby achieving the purpose of high-selectivity detection of hydrogen sulfide in a living body. The hydrogen sulfide and the silver nanoparticles are combined to form silver sulfide which is deposited to cause potential change on the surface of the electrode, and the potential change in the reaction process is monitored in real time through an open-circuit potentiometric method, so that the change of the hydrogen sulfide concentration can be reflected. Therefore, the method provided by the invention can realize high-selectivity detection of hydrogen sulfide under the condition closest to physiological conditions.

Description

technical field [0001] The invention belongs to the field of analysis, in particular to the preparation of nanometer silver carbon fiber microelectrodes pretreated with sodium sulfide and its application in detecting hydrogen sulfide in biological samples such as living brain nervous system. Background technique [0002] Hydrogen sulfide exists widely in organisms, participates in a variety of physiological and pathological processes and plays an important role, and is considered to be an important gas signal molecule. The content of hydrogen sulfide is closely related to the body's homeostasis; the abnormal (increased or decreased) content of hydrogen sulfide has an important relationship with cardiovascular diseases, endocrine disorders, gastrointestinal diseases and nervous system diseases. Therefore, it is of great significance to accurately monitor the hydrogen sulfide content in biological samples in real time. Common biological samples include cells (single cells, ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B5/1473
CPCA61B5/1473A61B5/14546A61B5/14507
Inventor 张美宁张丽
Owner RENMIN UNIVERSITY OF CHINA
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