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Method for identifying turmeric varieties by applying wide targeted metabonomics technology

A technology of metabolomics and turmeric, applied in the field of identification of turmeric varieties, can solve problems such as difficult to meet market demand, low output of Sichuan turmeric, etc., achieve the effect of promoting industrial development, solving the problem of short supply and high content

Active Publication Date: 2022-07-22
中国医学科学院药用植物研究所云南分所 +1
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Problems solved by technology

To sum up, Sichuan Qianwei is the authentic production area of ​​turmeric with the best quality. However, the output of Sichuan turmeric is not large and it is difficult to meet the market demand. Huge market demand for turmeric

Method used

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  • Method for identifying turmeric varieties by applying wide targeted metabonomics technology
  • Method for identifying turmeric varieties by applying wide targeted metabonomics technology
  • Method for identifying turmeric varieties by applying wide targeted metabonomics technology

Examples

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Embodiment 1

[0025] In this example, the turmeric to be identified is named as high-content turmeric.

[0026] A method to identify turmeric varieties using broad-targeted metabolomic technology includes the following steps:

[0027](1) Preparation of turmeric samples: collecting cloud turmeric, sichuan turmeric and fresh rhizomes of turmeric in the mature stage with high content of turmeric, operating in the dark, cut into uniform thin slices, dried in an oven at 35 ° C, pulverized and passed through a 60-mesh sieve, Take 1 g of turmeric powder in a 10 mL centrifuge tube, add 10 mL of methanol aqueous solution with a concentration of 80% by volume, shake for 1 min, ultrasonically extract for 10 min, centrifuge at 12,000 rpm for 5 min, separate the supernatant, and use 10 mL of 50% concentration of turmeric for the residue in turn. The methanol aqueous solution and 10 mL methanol were extracted once respectively, the three extracts were mixed, 1 mL of the extract was added to methanol to d...

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Abstract

The invention discloses a method for identifying turmeric varieties by applying a wide targeting metabonomics technology. The method comprises the following steps: preparing a turmeric sample and a mixed standard substance; detecting and analyzing a turmeric sample; performing qualitative and quantitative analysis on a turmeric sample; processing and analyzing metabonomics data; screening metabolites with significant differences; a target metabolite is subjected to normalization processing, a clustering heat map is drawn through MetaboAnalyst5.0 online software, the change condition of the metabolite is reflected, and the characteristic difference metabolite of different varieties of turmeric is screened out. According to the method for identifying the turmeric variety by applying a wide targeted metabonomics technology, a new variety of turmeric is found, an excellent strain with high yield and high content is found and cultivated, the development of the turmeric industry is promoted, the problem of short supply of the turmeric medicinal material is solved, and a new choice can be provided for the medicinal material source of the medicinal turmeric.

Description

technical field [0001] The invention belongs to the technical field of identification of turmeric varieties, and more specifically relates to a method for identification of turmeric varieties by using broad-targeted metabolomics technology. Background technique [0002] The 2020 edition of the "Chinese Pharmacopoeia" records that turmeric is the dried rhizome of Curcuma Longa L., a plant belonging to the genus Zingiberaceae. It is used for the treatment of tingling pain in the chest and flank, pain in the chest and heart, dysmenorrhea, dysmenorrhea, rheumatism, shoulder and arm pain, swelling and pain due to falling and fluttering. The active components of turmeric mainly include curcuminoids and volatile oils. Up to now, researchers have isolated more than 30 natural curcumin compounds from turmeric, mainly including curcumin, demethoxycurcumin, bis-demethoxycurcumin, etc. Among them, curcumin has the highest content and is the main Active ingredient. Pharmacological stu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/72G01N30/86
CPCG01N30/02G01N30/72G01N30/06G01N30/34G01N30/8675
Inventor 张丽霞罗祖良郭芳马小军唐德英
Owner 中国医学科学院药用植物研究所云南分所
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