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Testing method for monitoring microorganism transmission of indoor humidifying device

A technology for humidifying devices and testing methods, which is applied in the field of monitoring microorganisms transmitted by aerosols and in the field of testing microorganisms transmitted by indoor humidifying devices. The effect of stable data and low cost

Pending Publication Date: 2022-07-15
广州市微生物研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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No research report or patent application was found on the test method for monitoring the transmission of microorganisms in the humidification device after reviewing

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  • Testing method for monitoring microorganism transmission of indoor humidifying device
  • Testing method for monitoring microorganism transmission of indoor humidifying device
  • Testing method for monitoring microorganism transmission of indoor humidifying device

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Embodiment 1

[0031] A test method for monitoring the spread of microorganisms in an indoor humidification device, comprising the steps of:

[0032] S1. Preparation of the bacterial suspension of the target strain and preparation for the test: the materials and reagents used in the test are all used after high temperature sterilization; the test bacteria select the fourth generation Staphylococcus albus 8032, nutrient agar medium (NA), phosphate buffer solution ( PBS), a disposable flat plate (∅90 mm), 2 electronic balances (accuracy 0.1 g), 2 sets of 30 m3 test chambers (10,000-grade cleanliness), and a desktop evaporative humidification device (water tank capacity is 500 mL) . Inoculate Staphylococcus albicans 8032 in an appropriate amount of PBS to prepare a 100 mL initial bacterial suspension with a concentration of 1.3 × 10 7 cfu / mL;

[0033]S2. Site layout in the test chamber: place the humidification device and CN60Collison microbial aerosol generator on the balance and on the wor...

Embodiment 2

[0040] A test method for monitoring the spread of microorganisms in an indoor humidification device, comprising the steps of:

[0041] S1. Preparation of target strain bacterial suspension and test preparation: the test materials and reagents are used after high temperature sterilization; the test bacteria select the fourth generation of Aspergillus niger ATCC 16404, malt extract agar medium (MEA), Tween 80 Physiological saline, disposable flat plate (∅90 mm), 2 electronic balances (accuracy 0.1 g), 2 sets of 30 m3 test chambers (10,000-level cleanliness), and a floor-standing evaporative humidifier (water tank capacity is 2 L) ). The Aspergillus niger ATCC 16404 was scraped, filtered and centrifuged to prepare a 120 mL initial bacterial suspension with a concentration of 1.2×10 7 cfu / mL;

[0042] S2. Site layout in the test chamber: place the humidification device and CN60Collison microbial aerosol generator on the balance and place them on the ground, and then place them i...

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Abstract

The invention discloses a test method for monitoring air microorganisms transmitted by an indoor humidifying device. The test method comprises the following steps: S1, preparing a bacterial suspension of a target strain; s2, arranging a site in the test cabin; s3, background control of temperature, humidity and cleanliness of the test cabin and the contrast cabin; s4, operating a humidifying device in the test cabin, and operating a microbial aerosol generator in the contrast cabin; s5, after stirring and standing, respectively collecting the concentration of microorganisms in the test cabin and the contrast cabin through a liquid impact type microorganism sampler and a flat plate; and S6, respectively and properly diluting, culturing and counting the collected samples, and calculating the bacterium release inhibition efficiency of the humidifying device. By introducing the test chamber and the blank control chamber, natural attenuation generated in the transmission process of aerosol exposed in air can be ignored, the uncertainty of the experiment is greatly reduced, the objectivity and authenticity of the detection conclusion are fully ensured, and a more intuitive reference basis is provided for evaluation and supervision.

Description

technical field [0001] The invention relates to the field of biological analysis and testing, in particular to a testing method for monitoring aerosol-borne microorganisms, in particular to a testing method for monitoring microorganisms propagated by indoor humidification devices. Background technique [0002] At present, there are many reports on the safety problems caused by secondary pollution caused by the transmission of microorganisms in air conditioners, humidifiers and other devices at home and abroad, among which humidification devices are the most serious. In my country, humidification devices are widely used in various places, and have been integrated into all aspects of people's lives. We look at our lives everywhere, from small bedrooms and living rooms to large greenhouses and factories. Humidification devices can be seen everywhere. According to market research data, the sales volume of humidifiers will skyrocket from around October every year in my country,...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12Q1/06C12M1/34C12M1/38C12M1/36C12M1/00C12M1/02C12M1/28C12R1/01C12R1/645
CPCC12Q1/04C12Q1/06C12M41/12C12M41/48C12M41/00C12M27/02C12M41/36C12M23/00
Inventor 徐国洋黄东浪万分龙陈琼廖文锋张家云黄永良郑苗庄萍华曹万荣杜少平黄魁英
Owner 广州市微生物研究所有限公司
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