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Process for rapidly removing moisture in sialic acid product with low energy consumption

A sialic acid and low energy consumption technology, applied in the field of sialic acid preparation, can solve the problems of high energy consumption, affecting the quality of sialic acid powder products, and incomplete removal.

Pending Publication Date: 2022-07-15
德元堂(上海)健康科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods not only consume a lot of energy, but also are not completely removed. If heated for a long time, it is easy to affect the quality of the obtained sialic acid powder product.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A process for rapidly removing moisture from sialic acid products with low energy consumption, comprising the steps of:

[0024] (1) The Escherichia coli fermentation broth containing polysialic acid is filtered to remove solid substances such as mycelium and fermentation substrate, and the filtrate is collected. Then, 5 times the volume of ethanol is added to the filtrate, and after the precipitation is complete, the precipitation is separated by filtration to obtain the crude polysialic acid.

[0025] (2) Dissolving the crude polysialic acid in deionized water, then adding 0.1 mol / L hydrochloric acid to adjust pH=2 of the dissolving solution, then heating to 80° C. for hydrolysis reaction for 3 hours. After completion, sodium hydroxide is added to the obtained hydrolyzate to adjust the pH to be neutral to obtain a neutral hydrolyzate.

[0026] (3) adding activated carbon to the neutral hydrolyzate for decolorization treatment, and the added mass of the activated carb...

Embodiment 2

[0029] A process for rapidly removing moisture from sialic acid products with low energy consumption, comprising the steps of:

[0030] (1) The Escherichia coli fermentation broth containing polysialic acid is filtered to remove solid substances such as mycelium and fermentation substrate, and the filtrate is collected. Then, 4 times the volume of ethanol is added to the filtrate, and after the precipitation is complete, the precipitation is separated by filtration to obtain the crude polysialic acid.

[0031] (2) Dissolving the crude polysialic acid in deionized water, then adding 0.1 mol / L hydrochloric acid to adjust pH=1 of the dissolving solution, then heating to 85° C. for hydrolysis reaction for 2 hours. After completion, potassium hydroxide is added to the obtained hydrolyzate to adjust the pH to be neutral to obtain a neutral hydrolyzate.

[0032] (3) adding activated carbon to the neutral hydrolyzate for decolorization treatment, and the added mass of the activated c...

Embodiment 3

[0035] A process for rapidly removing moisture from sialic acid products with low energy consumption, comprising the steps of:

[0036] (1) The Escherichia coli fermentation broth containing polysialic acid is filtered to remove solid substances such as mycelium and fermentation substrate, and the filtrate is collected. Then, 5.5 times the volume of ethanol is added to the filtrate, and after the precipitation is complete, the precipitation is separated by filtration to obtain the crude polysialic acid.

[0037] (2) Dissolving the crude polysialic acid in deionized water, then adding 0.1 mol / L sulfuric acid to adjust pH=2 of the dissolving solution, then heating to 70° C. for hydrolysis reaction for 3 hours. After completion, potassium hydroxide is added to the obtained hydrolyzate to adjust the pH to be neutral to obtain a neutral hydrolyzate.

[0038] (3) adding activated carbon to the neutral hydrolyzate for decolorization treatment, and the added mass of the activated car...

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PUM

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Abstract

The invention discloses a low-energy-consumption technology for rapidly removing moisture in a sialic acid product, which comprises the following steps: (1) dissolving a polysialic acid crude product with deionized water, then carrying out hydrolysis reaction under acidic and heating conditions, and after the hydrolysis reaction is completed, adjusting the pH value of hydrolysate to be neutral to obtain neutral hydrolysate; and (2) decolorizing the neutral hydrolysate, adding ethanol into the obtained decolorized solution, and separating out the precipitate after the precipitate is completely precipitated, thereby obtaining a sialic acid crude product. (3) re-dissolving the sialic acid crude product in deionized water, then adding ethanol, separating out the precipitate after complete precipitation to obtain a wet sialic acid precipitate, then adding ethanol again, adjusting the ratio of the ethanol to the moisture in the wet sialic acid precipitate to reach an azeotropic ratio, then heating to azeotropic temperature until the ethanol and the moisture are removed, and thus obtaining the sialic acid crude product. The sialic acid powder is obtained. According to the process disclosed by the invention, the moisture can be removed at a lower temperature by utilizing the characteristic that the moisture and the ethanol in the wet sialic acid product are azeotropic.

Description

technical field [0001] The invention relates to the technical field of sialic acid preparation, in particular to a process for rapidly removing moisture from a sialic acid product with low energy consumption. Background technique [0002] The scientific name of sialic acid is N-acetylneuraminic acid, which is an important part of gangliosides in the brain, which has the effect of "inducing" and preventing the invasion of bacteria; it is usually located in the sugar part of the outermost layer of the cell membrane and secreted The key position of the sugar complex is an important material basis for the diversification of the structure and function of the sugar complex. At present, the preparation of sialic acid mainly adopts the microbial fermentation method, that is, the fermentation and metabolism of microorganisms such as Escherichia coli are used to generate polysialic acid, and then the polysialic acid is hydrolyzed into sialic acid monomers to prevent the preparation of...

Claims

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Application Information

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IPC IPC(8): C07H7/033C07H13/04C07H1/00C07H1/06
CPCC07H7/033C07H13/04C07H1/00C07H1/06
Inventor 卢健行刘长峰李光贤
Owner 德元堂(上海)健康科技发展有限公司
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