Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

SNP (Single Nucleotide Polymorphism) site for detecting watermelon peripheral leaf shape, closely-linked molecular marker and application of SNP site and closely-linked molecular marker

A molecular marker, watermelon technology, applied in the field of genetic engineering, can solve problems such as unreported, and achieve the effect of good application value

Active Publication Date: 2022-07-12
HENAN AGRICULTURAL UNIVERSITY
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] With the completion of the whole genome sequencing of watermelon, related research on watermelon has developed rapidly in recent years, many excellent traits have been gene mapped, and some linkage markers developed have also begun to be used in the process of molecular marker-assisted breeding. The study of tightly linked molecular markers for whole leaf shape of watermelon has not been reported yet

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • SNP (Single Nucleotide Polymorphism) site for detecting watermelon peripheral leaf shape, closely-linked molecular marker and application of SNP site and closely-linked molecular marker
  • SNP (Single Nucleotide Polymorphism) site for detecting watermelon peripheral leaf shape, closely-linked molecular marker and application of SNP site and closely-linked molecular marker
  • SNP (Single Nucleotide Polymorphism) site for detecting watermelon peripheral leaf shape, closely-linked molecular marker and application of SNP site and closely-linked molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1 Acquisition of SNP markers and closely linked dCAPS molecular markers co-segregated with the whole leaf shape gene of watermelon plants

[0060] This example mainly introduces the fine mapping process for the watermelon ClLL gene, including the construction of genetic segregation population, preliminary positioning, fine mapping and other processes (the analysis process is as follows: Figure 4 In this process, the development, design and inspection process of the final molecular markers dCAPS and SNP2 are involved. The relevant experimental process is briefly introduced as follows.

[0061] 1. Construction of genetically segregated populations

[0062] The watermelon whole leaf shape WT20 was used as the female parent, and the watermelon split leaf shape WT2 was used as the male parent (it needs to be explained that, during the experiment, considering the availability of materials and the convenience of operation, the inventors used WT2 as the male parent. O...

Embodiment 2

[0112] Example 2 Identification of watermelon as whole leaf-shaped material using dCAPS molecular markers

[0113] 1. DNA extraction from watermelon tissue

[0114] The DNA of the watermelon sample tissue was extracted by the conventional CTAB method, and the RNA was removed, and the volume of the DNA sample was not less than 50 μL. The OD values ​​of DNA samples at 260nm and 280nm were measured by UV spectrophotometer, and the DNA content and the ratio of OD260 / 280 were calculated. DNA sample purity OD260 / 280 value should be 1.8-2.0, and the concentration should be diluted to 100ng / μL.

[0115] 2. Primer selection

[0116] dCAPS2 uses the following primer sequences:

[0117] dCAPS2-F: 5'-AATGGGTATGATATTGTCCATCTC-3' (as shown in SEQ.ID.NO.1)

[0118] dCAPS2-R: 5'-AAAGTCTTGCATTGGCTAAAGA-3' (as shown in SEQ.ID.NO.2)

[0119] dCAPS3 uses the following primer sequences:

[0120] dCAPS3-F: 5'-AAAATTTGCATGTTTGGATTACAAAACTCA-3' (as shown in SEQ.ID.NO.3)

[0121] dCAPS3-R: 5'-T...

Embodiment 3

[0138] Example 3 Identify whether watermelon is whole leaf-shaped material using SNP molecular markers

[0139] 1. DNA extraction from watermelon tissue

[0140] The DNA of the watermelon sample tissue was extracted by the conventional CTAB method, and the RNA was removed, and the volume of the DNA sample was not less than 50 μL. The OD values ​​of DNA samples at 260nm and 280nm were measured by UV spectrophotometer, and the DNA content and the ratio of OD260 / 280 were calculated. DNA sample purity OD260 / 280 value should be 1.8-2.0, and the concentration should be diluted to 100ng / μL.

[0141] 2. Primer selection

[0142] SNP2 uses the following primer sequences:

[0143] SNP2-F: 5'-CCATTTTAGAATCACTCCCAAAC-3' (as shown in SEQ.ID.NO.9)

[0144] SNP2-R: 5'-AACTAAGCAAGAAGACATGTGACC-3' (as shown in SEQ.ID.NO.10)

[0145] The primers were synthesized by a biotechnology company and diluted to 10 μM before use.

[0146] 3. PCR reaction system

[0147] The PCR reaction program w...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of gene engineering, and particularly relates to an SNP (Single Nucleotide Polymorphism) site for detecting a watermelon peripheral leaf shape, a closely linked molecular marker and application. The molecular marker provided by the invention can identify the watermelon leaf shape character from the molecular level, can be directly used for molecular marker-assisted breeding of a watermelon peripheral leaf shape material, improves the breeding selection efficiency, and accelerates the breeding process; in addition, a physical map between the two molecular markers can be obtained by utilizing the molecular markers, so that a foundation is laid for cloning of the final watermelon peripheral leaf shape ClLL gene and establishment of a molecular marker assisted breeding system, and a foundation is also laid for research of a watermelon leaf development regulation network.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and particularly relates to a pair of molecular markers used for locating the whole leaf shape gene ClLL of watermelon and closely linked with the whole leaf shape gene ClLL of watermelon, and SNP sites for detecting the whole leaf shape of watermelon and applications. Background technique [0002] Selection is one of the most important links in breeding. It refers to the selection of genotypes that meet the requirements in a population for subsequent breeding. However, in the traditional breeding process, the basis of selection is often determined by the phenotype of the plant. Such selection is often time-consuming and may deviate from the genotype, resulting in inaccurate selection and low efficiency. The use of molecular marker-assisted breeding can quickly detect target genes or loci closely linked to target traits, and achieve the purpose of selecting target traits. [0003] T...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12Q1/6869C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 杨路明豆峻岭杨森朱华玉刘东明牛欢欢段世享
Owner HENAN AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com