Preparation method and application of marine fish oligopeptide capable of resisting fatigue and reducing uric acid

An oligopeptide, marine technology, applied in the biological field, can solve the problem of low development and utilization of marine fish protein, and achieve the effect of promoting high-value utilization, reducing uric acid generation and small molecular weight

Active Publication Date: 2022-07-12
GUANGDONG OCEAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the development trend of the above-mentioned active peptide with dual functions of anti-fatigue and uric acid reduction and the low development and utilization of marine fish protein, the present invention provides a The preparation method and application of marine fish oligopeptides with dual effects of anti-fatigue and uric acid reduction, exploring the high-value utilization of marine fish protein sources and the development of new active peptides with dual functions of anti-fatigue and uric acid reduction

Method used

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  • Preparation method and application of marine fish oligopeptide capable of resisting fatigue and reducing uric acid
  • Preparation method and application of marine fish oligopeptide capable of resisting fatigue and reducing uric acid
  • Preparation method and application of marine fish oligopeptide capable of resisting fatigue and reducing uric acid

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Embodiment 1

[0049] Example 1. Preparation and evaluation of Far Eastern sardine enzymolysate

[0050] (1) Preparation of Enzymatic Hydrolysate of Pseudo-Sardines from the Far East

[0051] Autolytic enzymolysis process: using fresh Far Eastern sardines ( Sardinops sagax ) (purchased from Guangdong Xingyi Marine Biological Engineering Co., Ltd.) as raw materials, remove the internal organs, scales and gills, harvest the meat, and grind it into surimi. Accurately weigh 500.00g of surimi, add distilled water at a ratio of 1:2 (g / mL) to liquid, homogenize, adjust pH to 6, temperature at 50 °C, and enzymatic hydrolysis for 3 h. Then, the enzyme was inactivated in a boiling water bath at 100 °C for 15 min, cooled, centrifuged at 4 °C, 8000 r / min for 20 min, and the supernatant was decolorized with activated carbon for 30 min to obtain the Far Eastern sardine enzymatic hydrolysate ( Sardinops sagax hydrolysate, SSH).

[0052] Enzymatic hydrolysis process of exogenous enzymes: Accurately we...

Embodiment 2

[0095] Embodiment 2, the preparation of flat rudder bonito enzymolysate

[0096] (1) Preparation and evaluation of the enzymatic hydrolyzate of bonito flakes

[0097] Autolytic enzymatic hydrolysis process: Using fresh flat rudder bonito (purchased from Guangdong Xingyi Marine Bioengineering Co., Ltd.) as raw material, remove the internal organs, scales and gills, harvest the meat, and grind it into surimi. Accurately weigh 500.00 g of surimi, add distilled water according to the ratio of material to liquid 1:3 (W:V, g / mL), homogenize, adjust the pH to 7, the temperature is 60 ° C, and enzymatic hydrolysis for 4 h. Then, the enzyme was inactivated in a boiling water bath at 100 °C for 15 min, cooled, centrifuged at 4 °C, 8000 r / min for 20 min to obtain the supernatant, and the supernatant was decolorized with activated carbon for 30 min to obtain the enzymatic hydrolyzate of bonito flakes ( Auxis thazard hydrolysate, ATH).

[0098]Exogenous enzyme enzymolysis process: Accur...

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Abstract

The invention discloses a preparation method and application of marine fish oligopeptide capable of resisting fatigue and reducing uric acid. The preparation method of the marine fish oligopeptide provided by the invention comprises the following steps: (1) mincing pretreated marine fish meat into fish paste, adding distilled water into the fish paste, homogenizing, adjusting the pH value, carrying out autolysis enzymolysis, and adding exogenous protease to carry out synergistic enzymolysis, so as to obtain a zymolyte; and (2) carrying out enzyme deactivation and centrifugation on the zymolyte, taking supernate, decoloring the supernate, filtering through a ceramic membrane and an ultrafiltration membrane, concentrating and drying to obtain the marine fish oligopeptide. The marine fish peptide with the dual effects of resisting fatigue and reducing uric acid has the functions of improving the fatigue state and resisting hyperuricemia, and a new thought is provided for high-valued utilization of marine fishes and development of novel peptide products.

Description

technical field [0001] The invention relates to the field of biology, in particular to a preparation method and application of a marine fish oligopeptide with both anti-fatigue and uric acid lowering properties. Background technique [0002] In the process of continuous high-intensity physical and mental work, if you do not adjust the rest in time, it will lead to physical and mental fatigue. Fatigue is a sub-health state, which can cause biological regulation disorders, autonomic disorders, and various diseases of the endocrine and immune systems. WHO's survey results show that fatigue has become a worldwide problem, and the number of people in a state of fatigue in the world accounts for more than 35% of the total number of people. The occurrence of fatigue reduces people's quality of life and work efficiency, and exacerbates the occurrence of sleep disorders and depression. Fatigue that cannot be recovered by rest is a sign of an underlying pathology or chronic disease....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34A61K38/01A61P39/00A61P19/06
CPCC12P21/06C07K1/34A61K38/012A61P39/00A61P19/06Y02P20/10
Inventor 吉宏武韦柳益瞿瑜杉张迪宋文奎苏伟明刘书成
Owner GUANGDONG OCEAN UNIVERSITY
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