Compound microbial agent for acid soil remediation as well as preparation method and application of compound microbial agent
A technology of compound microbial inoculants and microbial inoculants, which can be used in application, land preparation methods, fertilizer mixtures, etc., can solve problems such as quality degradation, soil alkalinity enhancement, soil acidification, etc.
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Embodiment 1
[0022] Preparation of microbial inoculum fermentation broth:
[0023] Preparation of Bacillus subtilis fermentation broth:
[0024] The seed liquid of Bacillus subtilis is inoculated in the fermentation medium (the inoculation amount is 5%), and the Bacillus subtilis fermentation liquid is obtained after the fermentation is carried out under the temperature condition of 25°C, and the number of effective viable bacteria in the fermentation liquid is ≥ 1 billion / The culture medium is composed of the following raw materials: 20g / L glucose, 15g / L peptone, 5g / L sodium chloride, 0.5g / L beef extract, and the balance is water.
[0025] Preparation of Bacillus terpenoids fermentation broth:
[0026] Inoculate the seed liquid of Brevibacterium lateralsporum into the fermentation medium (the inoculation amount is 5%), and ferment and culture at a temperature of 39 ° C to obtain the fermentation liquid of Brevibacterium lateralisporum, and the number of effective viable bacteria in the ...
Embodiment 2
[0028] Preparation of food waste fermentation residue
[0029] After sorting and crushing the kitchen waste, add water 1-3 times the weight of the kitchen waste to adjust the water content of the fermentation system, and then add calcium carbonate to keep the pH value of the fermentation system at 5-6; inoculation 5 After anaerobic fermentation of -7% lactic acid bacteria at 45°C, filter residues are obtained by filtration and separation; kitchen waste fermentation residues can be obtained by drying the filter residues at low temperature; the kitchen wastes are catering residues collected by municipalities; the Food waste is food and beverage leftovers collected by municipalities;
Embodiment 3
[0031] A composite microbial inoculant for acid soil remediation is prepared from the following raw materials in parts by weight: 20 parts of composite minerals, 10 parts of kitchen waste fermentation residue, 12 parts of egg shells, 10 parts of microbial inoculum fermentation liquid, 8 parts of sodium alginate and 4 parts of gluten are composed of; the composite minerals are composed of magnesia clay, medical stone, and phosphate rock, and the weight ratio of the described magnesia clay, medical stone, and phosphate rock is 1:1:1; the microbial inoculum fermentation broth is composed of Bacillus subtilis and Brevibacterium lateralis; the number of effective viable bacteria in the fermentation broth is ≥1 billion / g, and the exopolysaccharide is ≥1.0 mg / g; Bacillus subtilis The ratio of Bacillus to Brevibacterium lateralis is 2:1;
[0032] The preparation method of the described composite microbial inoculum for acid soil remediation is specifically:
[0033] Place the describe...
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