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Three-dimensional paper-based micro-fluidic chip for rapidly detecting ferric iron and preparation process of three-dimensional paper-based micro-fluidic chip

A microfluidic chip, ferric technology, applied in measuring devices, laboratory containers, material analysis by observing the impact on chemical indicators, etc., can solve the problem of high requirements for operators, increased detection costs, The operation steps are cumbersome and other problems, so as to save the test time, reduce the detection cost and widen the detection range.

Inactive Publication Date: 2022-07-08
SICHUAN AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods have high detection accuracy, they have disadvantages such as large equipment footprint, complicated operation process, high detection cost, high requirements for operators, and large amount of detection reagents.
In order to solve the two major problems currently existing in the above methods: the existing detection method is cumbersome in the detection process, requires the use of large instruments, and cannot achieve on-site instant detection; the existing detection method needs to be configured with a large number of detection reagents, not only the detection time Long, and the waste liquid needs to be treated, which increases the detection cost
The existing ferric ion detection methods have not applied paper-based microfluidic technology to the detection of ferric ion concentration in urban park water environment

Method used

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  • Three-dimensional paper-based micro-fluidic chip for rapidly detecting ferric iron and preparation process of three-dimensional paper-based micro-fluidic chip
  • Three-dimensional paper-based micro-fluidic chip for rapidly detecting ferric iron and preparation process of three-dimensional paper-based micro-fluidic chip
  • Three-dimensional paper-based micro-fluidic chip for rapidly detecting ferric iron and preparation process of three-dimensional paper-based micro-fluidic chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Screening of adsorption diffusion layer filter paper

[0062] Step 1: First use a household embossing machine and an octagonal plum-shaped carbon die No.1, No.3 and medium-speed filter paper (commercially available double-circle brand medium-speed filter paper with a diameter of 9 cm) were cut into octagonal plum-shaped paper chips.

[0063] Step 2: Use a household embossing machine and an 8mm round carbon die No.1 is cut into a circular color developing layer of 8mm. The circular color developing layer was soaked in 1 g / L ellagic acid solution for 20 minutes, taken out, placed on a digital display constant temperature heating plate, and dried at 100 °C until there was no moisture. Then follow figure 2 Sequential assembly into a three-dimensional paper-based microfluidic chip.

[0064] Step 3: Add 100 μL of Fe(III) solution with a concentration of 1 mg / L dropwise from the sample hole, wait for the Fe(III) solution to diffuse into the chromogenic layer...

Embodiment 2

[0068] Example 2 Screening of chromogenic layer filter paper

[0069] Step 1: According to the best liquid diffusion and color development selected in Example 1 No.1, then made into plum-shaped paper chips. Reuse home-type embossing machine and carbon tool model will No.1, No.3 and medium speed filter papers are cut into 6mm and 8mm round color developing layers.

[0070] Step 2: Soak all the round color developing layers in 1g / L ellagic acid solution for 20min, take them out, put them on a digital display constant temperature heating plate, and dry them at 100°C until there is no moisture. Then follow figure 2 The sequential assembly method in Table 1 and the combined manner of Table 1 into a three-dimensional paper-based microfluidic chip (Table 1).

[0071] Table 1

[0072]

[0073] Step 3: Add 100 μL of Fe(III) solution with a concentration of 1 mg / L dropwise from the sample addition hole. After the Fe(III) solution diffuses into the chromogenic layer and react...

Embodiment 3

[0075] Embodiment 3 Color development solution concentration screening

[0076] Step 1: according to the experimental results of Examples 1 and 2, cut plum-shaped paper chips ( No.1) and the circular color developing layer ( No.3). Ellagic acid solutions were then prepared at concentrations of 0.0, 0.5, 1.0, 2.0, 4.0, 6.0, 8.0, 10.0, 12.0, 15.0 and 20.0 g / L.

[0077] Step 2: Soak the circular color developing layer in the ellagic acid solution of 0.0, 0.5, 1.0, 2.0, 4.0, 6.0, 8.0, 10.0, 12.0, 15.0 and 20.0g / L for 20min respectively, then take it out, put it on the digital display Dry on a constant temperature heating plate at 100°C until there is no moisture. Then follow figure 2 3D paper-based microfluidic chips are assembled by the sequential assembly method in .

[0078] Step 3: Add 100 μL of Fe(III) solution with a concentration of 1 mg / L dropwise from the sample hole, wait for the Fe(III) solution to diffuse into the chromogenic layer and react with ellagic acid a...

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Abstract

The invention relates to the technical field of test paper strips, in particular to a three-dimensional paper-based micro-fluidic chip for rapidly detecting ferric iron and a preparation process of the three-dimensional paper-based micro-fluidic chip. Comprising an isolating layer on the bottom layer, a color developing layer arranged on the isolating layer, an adsorption diffusion layer arranged on the color developing layer and a cover plate layer arranged on the adsorption diffusion layer, wherein the color developing layer is No.3 filter paper adsorbed with a color developing agent, and the adsorption layer is No.1 filter paper; a sample adding hole is formed in the center of the cover plate layer. According to the method, on-site detection methods of the ferric iron Fe are enriched, and meanwhile, the ferric iron Fe in the urban park water environment is detected by adopting a low-carbon technology.

Description

technical field [0001] The invention relates to the technical field of test strips, in particular to a three-dimensional paper-based microfluidic chip for rapidly detecting ferric iron and a preparation process. Background technique [0002] With the continuous improvement of human productivity and the change of social structure, the focus of human life has gradually shifted from the industrialization era focusing on industrial and agricultural production to the post-industrial era focusing on spiritual civilization and leisure life. People look for green plants and places with abundant water resources and fresh air for recreation. As a place for urban residents to exercise, as a leisure and entertainment place that provides green plants and fresh air, urban parks are becoming more and more important in the construction of cities. [0003] The water body in the city park is one of the main components in the city park system, with a large area and strict water quality standa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/01B01L3/00
CPCG01N21/78G01N21/01B01L3/502707B01L3/50273B01L2300/0887
Inventor 罗鸿兵庄戴维梁洁张可刘晓玲江兵张笑笑李林莫忧安晓婵范义晨
Owner SICHUAN AGRI UNIV
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