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Preparation and application of streptomyces complex microbial inoculant

A technology of composite bacterial agent and Streptomyces, which is applied in the field of agricultural microorganisms, can solve the problems that Streptomyces products cannot be produced in large quantities, cannot be used in large-scale production, and it is difficult to form spores, etc., so as to expand the production of Streptomyces fermentation products, shorten Preparation time, effect of maintaining vitality

Pending Publication Date: 2022-07-08
JINAN JINNIU BIOTECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unlike spore products, it is difficult for Streptomyces to form spores in liquid. Therefore, it is impossible to use the mature liquid fermentation system on the market for large-scale production, and only the solid fermentation process can be used to produce Streptomyces spore preparations
At present, the solid fermentation process adopted by various companies in our country has the characteristics of large space occupation and small operation batches, which makes it impossible to produce Streptomyces products in large quantities.

Method used

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  • Preparation and application of streptomyces complex microbial inoculant
  • Preparation and application of streptomyces complex microbial inoculant
  • Preparation and application of streptomyces complex microbial inoculant

Examples

Experimental program
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Effect test

preparation example Construction

[0033] Preparation of liquid seed culture: Inoculate the activated colonies on the plate in liquid medium at 28°C, 200 rpm, and incubate for 48 hours.

[0034] Preparation of solid seed culture: The liquid seed culture is inoculated into the solid seed medium according to the proportion of 20%, and after 2-3 days of static culture at 28 degrees, the fermented material is shaken to make it fully mixed, and the culture is continued for 3-4 days. Fresh solid seed cultures can be obtained.

[0035] Three consecutive generations of solid seed cultures:

[0036] Preparation of solid fermentation culture: Inoculate the fresh solid seed culture in the solid fermentation medium according to the proportion of 10%, and after standing at 28 degrees for 2-3 days, stir the fermentation to make it fully mixed, and continue to culture for 3- 4 days, the solid fermentation culture that can be obtained after natural air-drying for 2 days, namely the bacterial agent of Streptomyces lividans W68...

Embodiment 1

[0046] Preparation of Streptomyces lividans W68 inoculum:

[0047] (1) Activation of W68: The strain was taken out from -80 degrees, streaked with MS medium, and cultured at 28 degrees for 5-6 days until a single colony was formed.

[0048] (2) Preparation of liquid seed culture: inoculate a single colony into a 500 mL Erlenmeyer flask containing 100 mL of 2×YT medium or 100 mL of NM medium, at 28°C, 200 rpm, and culture for 48 hours.

[0049] (3) Preparation of solid seed culture: the liquid seed culture is inoculated into the solid seed medium according to the proportion of 20%, and after standing at 28 degrees for 2-3 days, the fermented product is shaken to make it fully mixed, and the culture is continued for 3 days. - A fresh first generation of solid seed cultures can be obtained after 4 days.

[0050] (4) Continuous three generations of solid seed culture:

[0051](5) Preparation of solid fermentation culture: inoculate the fresh solid seed culture in the solid ferme...

Embodiment 2

[0053] Application Example of W68 Microbial Agent in Dong'e County, Shandong Province

[0054] The trial period is from January 2021 to April 2021.

[0055] The soil type of the experimental site is fluvo-aquic soil, the surface texture is medium soil, and vegetables have been grown for two consecutive years.

[0056] Application method: On the basis of conventional fertilization, spread the application before sowing the spinach and plough after the planting row.

[0057] Application rate: 500 grams of microbial inoculum per mu.

[0058] Experimental design: Control group: On the basis of farmers' routine fertilization, inactivated W68 microbial inoculants were applied. W68 inoculum treatment group: On the basis of the farmers' routine fertilization, 500 grams of W68 microbial inoculum with a spore content of 4 billion / g was applied. Production statistics at harvest on April 6, 2021 (Table 1).

[0059] The data shows that in this experiment, the application of Streptomyces...

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Abstract

The invention belongs to the field of agricultural microorganisms, and discloses preparation and application of a streptomyces complex microbial inoculant. According to the streptomyces albidoflavus W68 provided by the invention, seeds are prepared by adopting a liquid-solid two-phase method, wherein a solid seed culture can be continuously subcultured for three times. The prepared streptomyces albidoflavus microbial agent is compounded with bacillus to prepare the compound microbial agent, so that the compound microbial agent not only has the effect of preventing and treating plant diseases, but also can improve the yield, and has better economic benefits.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and mainly relates to the preparation and application of a compound Streptomyces agent. Background technique [0002] The information disclosed in this Background section is only for enhancement of understanding of the general background of the invention and should not necessarily be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art. [0003] Streptomyces species are known for producing abundant and structurally diverse secondary metabolites. Currently, live bacterial preparations or secondary metabolites of many Streptomyces species have been widely used in medicine, industry and agriculture. [0004] In my country's agricultural production, the largest number of Streptomyces species registered as microbial inoculants or microbial fertilizers are Streptomyces flavus, Streptomyces flexne...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/28A01N63/22A01P21/00A01P3/00C12R1/465C12R1/07C12R1/125
CPCC12N1/20A01N63/28A01N63/22
Inventor 张振颖黄俊李少杰张龙孙宪昀胡成成
Owner JINAN JINNIU BIOTECH CO LTD
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