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Active peptide for facial flora regulation and active peptide composition preparation method and application thereof

A technology of active peptides and compositions, applied in the field of biomedicine, can solve problems such as failure to take into account the microecological balance of the skin, and achieve excellent flora regulation activity, wide application prospects, and the effect of regulating facial flora.

Pending Publication Date: 2022-07-08
深圳海创生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most acne treatment agents only start from inhibiting the proliferation of Propionibacterium acnes and relieving local symptoms, without considering the balance of skin microecology

Method used

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  • Active peptide for facial flora regulation and active peptide composition preparation method and application thereof
  • Active peptide for facial flora regulation and active peptide composition preparation method and application thereof
  • Active peptide for facial flora regulation and active peptide composition preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1 provides a method for separating Saccharomyces cerevisiae cyclopeptide-I and Difibromyces cerevisiae cyclopeptide-II.

[0067] Mix 80g of yeast extract with 1000mL of ethyl acetate and 4000mL of water. After standing, extract to obtain an aqueous phase layer. Repeat the extraction process several times. The aqueous phase layer is lyophilized under vacuum and low temperature, and then prepared and separated by preparative HPLC. , to obtain Saccharomyces cerevisiae cyclopeptide-I (BOP-I). The yeast extract was purchased from Shenzhen Lusen Biotechnology Co., Ltd.

[0068] Mix 80 g of the lysate of the fermentation product of bifidida yeast (CAS number: 96507-89-0) with 1000 mL of ethyl acetate and 4000 mL of water. Freeze-drying at low temperature in vacuo, and then preparatively separated by preparative HPLC to obtain cyclopeptide-II (EOP-II).

[0069] The preparation conditions of the above-mentioned preparative HPLC are: take 0.1v% trifluoroacetic acid aque...

Embodiment 2

[0073] Example 2 provides a chemical synthesis method of Saccharomyces cerevisiae cyclopeptide-I and Difibromyces cerevisiae cyclopeptide-II.

[0074] (1) Take 100mg of Fmoc-Phe Wang Resin (Fmoc-Phe-Wang Resin) and put it in a solid-phase synthesis tube, add N,N-dimethylformamide (DMF), and let it stand to fully swell the resin, filter out solvent, and then piperidine DMF solution was added, and the solvent was filtered off after shaking. Fmoc-Trp-OH (CAS number: 35737-15-6), 1-hydroxybenzotriazole (CAS number: 2592-95-2), O-benzotriazole-tetramethylurea hexafluorophosphate The ester (CAS No.: 94790-37-1) was dissolved in DMF, and then N,N-diisopropylethylamine (CAS No.: 7087-68-5) was added and mixed well. It was added to the resin, stirred with nitrogen at room temperature, filtered with suction, washed with DMF and dichloromethane in turn, and the solvent was dried by suction. Repeat the above steps, mix the activated Fmoc-Leu-OH (CAS No.: 35661-60-0), Fmoc-His-OH (CAS No...

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PUM

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Abstract

The invention relates to the technical field of biomedicine, and particularly discloses an active peptide, an active peptide composition and application thereof. The amino acid sequence of the active peptide is as shown in SEQ ID No. 1 or SEQ ID No. 2. The active peptide composition comprises active peptides as shown in SEQ ID No. 1 and SEQ ID No. 2. The invention also discloses a preparation method of the active peptide composition. The active peptide or the active peptide composition disclosed by the invention has the effect of regulating facial flora; particularly, the composition has the effects of inhibiting the proliferation of staphylococcus epidermidis and propionibacterium acnes and promoting the proliferation of lactobacillus and bifidobacteria.

Description

technical field [0001] The present invention relates to the technical field of biomedicine, in particular to an active peptide for regulating facial flora, an active peptide composition and its application. Background technique [0002] The human body is a huge micro-ecological system, and a relatively stable micro-ecological environment is formed between skin bacteria and between bacteria and skin. The balance of this environment plays a vital role in maintaining skin health. The normal flora of the skin mainly includes three categories: the native flora, the transit flora and the symbiotic flora. The native flora is a type of resident flora represented by Propionibacterium acnes, which reside in the deep layers of the skin, hair follicles, sweat glands and sebaceous glands. They are the barrier to protect the human body from harmful microorganisms; passing flora, such as golden grapes Cocci, Escherichia coli and other bacterial and fungal flora, these flora are mostly pat...

Claims

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Application Information

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IPC IPC(8): C07K5/12A61K38/12A61K8/64A61Q17/00A61Q19/00A61P31/04A61P17/00A23L33/18
CPCC07K5/126A61K8/64A61Q17/005A61Q19/00A61P31/04A61P17/00A23L33/18A61K38/00A61K2800/5922A61K2800/85Y02A50/30
Inventor 刘志刚刘杰牛文芳王志尧
Owner 深圳海创生物科技有限公司
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