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Rape mutation breeding method

A technology for mutation breeding and rapeseed, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of narrow genetic background, high quality, high yield, difficult to combine multiple resistances, etc., and achieves early maturity and bacteria resistance. Effects of Sclerotinia Enhancement, Yield and Oil Quality Improvement

Pending Publication Date: 2022-07-01
ANHUI JINPEIYIN TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, Brassica napus is widely planted in my country. The germplasm resources are from Europe, and the genetic background is narrow. It is difficult to combine high quality, high yield and multi-resistance.

Method used

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Effect test

Embodiment 1

[0024] A method for mutation breeding of rapeseed, characterized in that, comprising the steps:

[0025] Step 1. Take the clone test tube seedlings cultivated from the young embryos of rapeseed, expand and multiply to form a large number of bud clusters, sterilize them with a sodium hypochlorite solution with a volume percentage of 5% for 25 minutes, wash them once with distilled water, and finally soak them in distilled water. embryo 1 hour;

[0026] In the present embodiment, the young embryos in step 1 are young embryos of Brassica napus 15 days after pollination.

[0027] Step 2. Under aseptic conditions, a large number of bud clusters were firstly connected to MS as a medium and then added to a mutagenesis medium of 0.3% ethyl methanesulfonate for 15 hours of mutagenesis;

[0028] Step 3. Then add a selection agent that has been filtered and sterilized by suction for selection, select MS as the culture medium, and add auxin in different proportions, and treat it by light...

Embodiment 2

[0040] A method for mutation breeding of rapeseed, characterized in that, comprising the steps:

[0041] Step 1. Take the clone test tube seedlings cultivated from the immature embryos of rapeseed, expand and multiply to form a large number of bud clusters, disinfect with 5% sodium hypochlorite solution by volume for 28 minutes, then wash twice with distilled water, and finally soak with distilled water. embryo 2 hours;

[0042] In the present embodiment, the young embryos in step 1 are Brassica napus young embryos 18 days after pollination.

[0043] Step 2. Under aseptic conditions, a large number of bud clusters were firstly connected to MS as a medium and then added to the mutagenesis medium of 0.3% ethyl methanesulfonate and cultured for 18 hours;

[0044] Step 3. Then add a selection agent that has been filtered and sterilized for selection, select MS as a culture medium, and add auxin in different proportions, and treat it by lighting under a fluorescent lamp, the light...

Embodiment 3

[0056] A method for mutation breeding of rapeseed, characterized in that, comprising the steps:

[0057] Step 1. Take the clone test tube seedlings cultivated from the immature embryos of rapeseed, expand and multiply to form a large number of bud clusters, disinfect with 5% sodium hypochlorite solution by volume for 30 minutes, then wash with distilled water 3 times, and finally soak with distilled water. embryo 4 hours;

[0058] In the present embodiment, the young embryos in step 1 are the young embryos of Brassica napus 20 days after pollination.

[0059] Step 2. Under sterile conditions, a large number of bud clusters were firstly connected to MS as a medium and then added to the mutagenesis medium of 0.3% ethyl methanesulfonate for 20 hours of mutagenesis;

[0060] Step 3. Then add a selection agent that has been filtered and sterilized by suction for selection, select MS as the culture medium, and add auxin in different proportions, and treat it by lighting under a flu...

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Abstract

The invention discloses an oilseed rape mutation breeding method, and relates to the technical field of oilseed rape breeding, a test-tube plantlet cultured by a cabbage type oilseed rape young blank is adopted for mutagenesis, firstly, MS is used as a culture medium and is added into a mutagenesis culture medium containing 0.3% of ethyl methanesulfonate for mutagenesis culture, then C5H9NO2 and C5H9NO3 are used for two rounds of screening, and the mutagenesis of the oilseed rape is realized. According to the present invention, the enzyme activity of the HMP pathway of the mutant screened by C5H9NO2 and C5H9NO3 is strong, and the determination result of the glucose-6-phosphate dehydrogenase activity in the rapeseed forming process proves that the synthesis of fatty acid is easily achieved, such that the yield and the oil content quality of the rape are improved, the sclerotiniose resistance is enhanced, and the mature period is advanced.

Description

technical field [0001] The invention relates to the technical field of rapeseed breeding, in particular to a rapeseed mutation breeding method. Background technique [0002] Rape includes three cultivars: Brassica napus, Cabbage-type rape and Mustard-type rape. At present, Brassica napus is widely planted in my country. The germplasm resources are from Europe, and the genetic background is narrow. It is difficult to combine the three factors of high quality, high yield and multi-resistance. Its single genetic basis has become a very prominent problem in rapeseed breeding work. Germplasm resources need to be expanded urgently. The lack of local and wild germplasm has prompted us to continuously create artificial germplasm resources through various means and approaches. Therefore, on the basis of extensive collection of rapeseed germplasm resources, advanced science and technology are used to innovate existing germplasm resources, broaden the genetic basis, obtain high-qualit...

Claims

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Application Information

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IPC IPC(8): A01H1/06A01H4/00
CPCA01H1/06A01H4/00A01H4/002
Inventor 王亚男樊友鑫范思静
Owner ANHUI JINPEIYIN TECH
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