Bacillus subtilis G-1 for producing organic acid and application thereof

A Bacillus subtilis, organic acid technology, applied in the field of microorganisms, can solve the problems of endogenous infection of livestock and poultry, residues of livestock products and the environment, double infection, etc.

Active Publication Date: 2022-06-28
河北谷旺金莱生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, the abuse of antibiotics has been very serious. The abuse of antibiotics will not only cause bacteria to develop drug resistance, but also reduce the immunity of livestock and poultry, causing endogenous infection and superinfection of livestock and poultry, and will also cause residues in livestock products and the environment.

Method used

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  • Bacillus subtilis G-1 for producing organic acid and application thereof
  • Bacillus subtilis G-1 for producing organic acid and application thereof
  • Bacillus subtilis G-1 for producing organic acid and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] 1.1 Isolation and purification of strains

[0051] Collect the whole plant corn, whole plant strawberry, and pine vanilla from the feed factory in Lianchi District, Baoding City, Hebei Province, package and ferment them for 3 to 5 days, take 10g of the fermented sample, add it to 100mL of sterile water, and place it at 37°C, Shake at 150 r / min for 1-2 hours, take out 1 mL of it and add it to a sterile test tube to dilute it, and then spread the plate and place it at 37°C for cultivation to obtain isolated strains. Finally, the isolated strains are purified to obtain purified strains.

[0052] 1.2 Identification of strain G-1

[0053] The strain obtained by the above separation and purification was named G-1, and it was coated on the NA medium for observation of colony morphology. The strain formed on the solid medium with an irregular shape, milky white, opaque, small folds, and slightly folds in the middle. Raised colonies, gram stained purple, are gram positive; spor...

Embodiment 2

[0056] 2.1 Study on growth characteristics of strain G-1

[0057] The strain G-1 was inoculated from the slant medium to the NA solid medium for activation, and the activated single colony was inoculated into 100 mL of seed medium, and cultured with shaking at 37 °C and 150 r / min for 6 to 12 hours; cultured to OD 600 The value is 0.4 to 0.6, which is the logarithmic growth phase of the strain, and the seed liquid is obtained.

[0058] 2.1.1 The effect of different temperatures on the growth of strain G-1

[0059] The activated strain seed liquid was inserted into the fermentation medium at an inoculum of 6.0%, and 3 replicates were set for each, and cultured at 10, 15, 20, 25, 30, 35, and 40°C respectively, and the growth of the strain was observed. , and the results are shown in Table 1.

[0060] Table 1 Growth characteristics of strain G-1 under different temperature conditions

[0061]

[0062] Note: "-" does not grow, "w" grows weakly, "+" grows, "++" grows better, a...

Embodiment 3

[0090] Effects of Strain G-1 on Whole Plant Corn Silage

[0091] The whole corn (Zheng Dan 958) planted in the experimental farm of Hebei Agricultural University was cut at the wax maturity stage, and transported to the forage processing base of Hebei Feed Microbial Technology Innovation Center, dried for half a day, and crushed with a silk rolling machine. The compound silage inoculant was added to the crushed whole corn, and the total amount of bacteria G-1 reached 1.0×10 9 CFU / kg (fresh weight), as a test group (T), silage was carried out in plastic bags with a size of 30cm×40cm, and the anaerobic fermentation was carried out by compaction for 45 days.

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus subtilis G-1 for producing organic acid and application of the bacillus subtilis G-1. According to the invention, a strain of bacillus subtilis G-1 with relatively strong organic acid production capacity is screened from whole corn which is naturally ensiled for 3-5 days, and is biologically preserved, and the preservation number is CGMCC No.21706. The strain still grows well under the conditions of 10 DEG C, pH 4.0 and 0.30% cholate, can produce lactic acid and acetic acid, has high acid production rate, and can be used for producing lactic acid and acetic acid. The bacillus subtilis has strong bacteriostatic activity on escherichia coli, salmonella and shigella dysenteriae, and also has strong bacteriostatic activity on saccharomycetes and molds causing feed deterioration.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to an organic acid-producing Bacillus subtilis G-1 and its application. Background technique [0002] With the rapid development of animal husbandry in recent years, the application of silage has become more and more extensive, and the quality requirements of silage are also getting higher and higher. In order to promote silage fermentation and improve the quality and stability of silage fermentation, it is often necessary to add some silage additives. Bacillus subtilis is non-toxic and harmless to humans and animals because of its characteristics of acid production and antibacterial substances. It has broad-spectrum antibacterial activity and strong resistance to stress, so it can be used as an silage additive. At the same time, because of its characteristics of easy production, low price, long storage time and easy transportation, using it as a silage additive ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/54C12P7/56A23K10/12A23K10/30A23K30/18A01N63/22A01P1/00A01P3/00C12R1/125
CPCC12P7/54C12P7/56A23K30/18A23K10/12A23K10/30A01N63/22Y02P60/87
Inventor 郭晓军伍常菁郭威田祖光范爱华朱宝成
Owner 河北谷旺金莱生物科技有限公司
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