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Application of miR-199a-3p in sperm in preparation of depression detection products and anti-depression drugs

A technology of mir-199a-3p and depression, which is applied in the field of biomedicine, can solve unclear problems and achieve the effects of improving accuracy, improving treatment effect, and high detection efficiency

Pending Publication Date: 2022-06-24
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In particular, it remains unclear whether the pathological symptoms of depression can be transmitted to the next generation through sperm RNA-mediated intergenerational inheritance, although paternal traumatic experiences and stress may adversely affect offspring through sperm RNA

Method used

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  • Application of miR-199a-3p in sperm in preparation of depression detection products and anti-depression drugs
  • Application of miR-199a-3p in sperm in preparation of depression detection products and anti-depression drugs
  • Application of miR-199a-3p in sperm in preparation of depression detection products and anti-depression drugs

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Experimental program
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Embodiment 1

[0047] This embodiment provides an application of miR-199a-3p in sperm in the preparation of a depression detection product.

[0048] Specifically, the nucleotide sequence of the miR-199a-3p is ACAGUAGUCUGCACAUUGGUUA.

[0049] The detection product uses the content of miR-199a-3p in sperm as a detection index, and when the content of miR-199a-3p is higher than the normal level of the same species, it indicates a risk. Preferably, the higher than the normal level of similar substances is higher than 2 times and above.

[0050] The miR-199a-3p in this embodiment can be at least one of initial miR-199a-3p, precursor miR-199a-3p, mature miR-199a-3p, initial miR-199a-3p and precursor miR -199a-3p can be cleaved and expressed as mature miR-199a-3p in human cells.

[0051] The miR-199a-3p in this embodiment can also include its variants, that is, functional equivalents of constitutive nucleic acid molecules, and the variants can display the same function as the miR-199a-3p nucleic ...

Embodiment 2

[0056] On the basis of Embodiment 1, this embodiment provides a depression detection product.

[0057] The depression detection product includes miR-199a-3p as described in Example 1.

[0058] The depression detection product can be a chip or a kit; the chip includes a solid phase carrier and an oligonucleotide probe immobilized on the solid phase carrier, and the oligonucleotide probe includes a specificity corresponding to the Part or all of the sequence of the miR-199a-3p; the kit includes reagents for detecting the expression level of the miR-199a-3p, the reagents include primers and / or probes for the miR-199a-3p.

[0059] The above-mentioned oligonucleotide probes may also include existing miR-199a-3p oligonucleotide probes capable of diagnosing depression or depression tendency. In practical applications, one, two or more detection probes for miR-199a-3p described above can be placed on the same chip for detection.

[0060] The above reagents may also include existing ...

Embodiment 3

[0063] This embodiment provides an application of miR-199a-3p in sperm in the preparation of antidepressant drugs. The antidepressant drug includes an inhibitor of the miR-199a-3p.

[0064] The nucleotide sequence of the miR-199a-3p is ACAGUAGUCUGCACAUUGGUUA.

[0065] Preferably, the inhibitor includes the reverse complement of miR-199a-3p (ie, capable of pairing with miR-199a-3p to form a double-stranded structure) or an inhibitor that inhibits replication or expression of miR-199a-3p.

[0066] In practical applications, the inhibitor of miR-199a-3p is used in combination with other agents that inhibit the expression of miR-199a-3p or attenuate the function of miR-199a-3p in the preparation of antidepressant drugs.

[0067] The application innovatively applies miR-199a-3p in sperm to antidepressant drugs, and suppresses the content of specific miR-199a-3p in sperm through miR-199a-3p inhibitor to weaken the heredity of depression, and to treat depression, It can effectively...

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Abstract

The invention provides an application of miR-199a-3p in sperm in preparation of a depression detection product and an anti-depression drug. According to the application, miR-199a-3p in sperms is innovatively applied to a depression detection product and an antidepressant drug, depression is detected and treated based on the specific miR-199a-3p in sperms, the depression detection product can effectively improve the accuracy of depression detection and is high in detection efficiency, and the antidepressant drug can improve the treatment effect of depression and can also improve the treatment effect of sperms. The intergenerational inheritance of depression can be effectively inhibited, and large-scale popularization and application are easy.

Description

technical field [0001] The present application relates to the field of biomedical technology, in particular to the application of miR-199a-3p in sperm in the preparation of depression detection products and antidepressant drugs. [0002] technical background [0003] Depression, also known as depressive disorder, is one of the most common and most disabling mental illnesses in the world. Currently, as many as 120-200 million people suffer from depression worldwide, and despite decades of research, the cause of depression remains a mystery. Current views on the causes of depression are the result of a combination of genetics and the environment. [0004] Existing research has shown that the environment can affect heredity. For example, children born and raised under the great famine are more prone to obesity, and the father's alcohol consumption may cause the fetus to develop alcohol syndrome symptoms. However, large gaps remain in our knowledge of genetic risk for depressio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883A61K45/00A61P25/24
CPCC12Q1/6883A61K45/00A61P25/24C12Q2600/158C12Q2600/178C12Q2600/136
Inventor 陈熹朱景宁张辰宇王延博陈张朋胡欢欢
Owner NANJING UNIV
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