Culture medium and method for inducing one-step seedling formation of cremastra appendiculata pseudobulb plants
A culture medium formula and culture medium technology, applied in botany equipment and methods, horticultural methods, applications, etc., can solve problems such as unstable inheritance of good traits, wildly different biological yields per plant, and restrictions on large-scale production. Achieve the effects of improving domestication survival rate, simplifying production steps, increasing differentiation and rooting rate
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Embodiment 1
[0027] A method for utilizing Rhododendron orchid pseudobulbs to induce plants to grow into seedlings in one step, the steps comprising:
[0028] 1. Explant selection and cleaning: Choose Rhododendron orchid, transplant it indoors, irrigate the roots with 75% (v / v) 500 times of carbendazim every 3 days, and irrigate the roots continuously for 4 times; After the root is completed, take the underground pseudobulb, remove the stems and leaves on the ground and the root system on the surface, rinse with saturated soapy water solution for 10 minutes, rinse with tap water 3 times, scrape off the raised and depressed parts of the epidermis with a knife, and then add 2 drops of Tween- 80, shake for 15 minutes, then rinse with tap water for 60 minutes;
[0029] 2. Disinfection of explants: transfer the washed explants to an ultra-clean workbench, disinfect with 75% (v / v) alcohol for 20s, rinse with sterile water for 2 times, and disinfect with saturated bleach solution for 18min, steri...
Embodiment 2
[0034] A method for utilizing Rhododendron orchid pseudobulbs to induce plants to grow into seedlings in one step, the steps comprising:
[0035] 1. Explant selection and cleaning: Choose Rhododendron orchid, transplant it indoors, irrigate the roots with 75% (v / v) 500 times of carbendazim every 3 days, and irrigate the roots continuously for 5 times; After the root is completed, take the underground pseudobulb, remove the stems and leaves on the ground and the root system on the surface, rinse it with saturated soapy water solution for 12 minutes, rinse it with tap water 4 times, scrape off the raised and depressed parts of the epidermis with a knife, and then add 3 drops of Tween- 80, shake for 13 minutes, then rinse with tap water for 70 minutes;
[0036] 2. Disinfection of explants: transfer the washed explants to an ultra-clean workbench, disinfect with 75% (v / v) alcohol for 25s, rinse with sterile water for 3 times, disinfect with saturated bleaching powder solution for ...
Embodiment 3
[0042] A method for utilizing Rhododendron orchid pseudobulbs to induce plants to grow into seedlings in one step, the steps comprising:
[0043] 1. Selection and cleaning of explants: Choose Rhododendron orchid, transplant them indoors, irrigate the roots with 500 times of 75% (v / v) carbendazim every 3 days, and irrigate the roots continuously for 5 times; After irrigating the roots, take the underground pseudobulbs, remove the stems, leaves and surface roots, rinse with saturated soapy water solution for 15 minutes, rinse with tap water 4 times, scrape off the raised and depressed parts of the epidermis with a knife, and then add 3 drops of Tween -80, shake for 15 minutes, and then rinse with tap water for 80 minutes.
[0044] 2. Disinfection of explants: transfer the washed explants to an ultra-clean workbench, disinfect with 75% (v / v) alcohol for 25s, rinse with sterile water for 3 times, disinfect with saturated bleaching powder solution for 20min, sterile Washing with w...
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