Inoculation identification method for peanut bacterial wilt in field initial flowering stage

Abstract

The invention discloses a peanut bacterial wilt field initial flowering stage inoculation identification method which comprises the following steps: carrying out soil preparation and ridging on a planting field, and planting peanut germplasm according to specifications; ralstonia solanacearum is selected to be cultured and shaken, and Ralstonia solanacearum suspension for inoculation is obtained; peanut germplasm materials in the initial flowering stage are inoculated with peanut ralstonia solanacearum through a leaf cutting inoculation method; and 25 days after inoculation, recording the number of plants with disease grades after inoculation of the ralstonia solanacearum, calculating disease indexes, and judging the resistance grade of the peanut variety to the peanut bacterial wilt. According to the inoculation identification method for the peanut bacterial wilt in the field initial flowering stage, the identification result is accurate and reliable, the repeatability is high, the bacterial wilt resistance in the field can be truly reflected, the resistance difference of different peanut varieties to the bacterial wilt can be remarkably distinguished, and large-batch identification can be performed on multiple to-be-detected varieties of peanuts at a time; the large-scale disease resistance identification of the peanut bacterial wilt is realized.

Description

technical field [0001] The invention belongs to the field of plant bacterial wilt resistance identification, in particular to a method for identification of peanut bacterial wilt inoculation at the early flowering stage in the field. Background technique [0002] Peanut is an important oilseed and economic crop in my country, and occupies an important position in the national production of our country. my country is a major producer of peanuts. In 2018, the sown area of ​​peanuts reached 4,620,000 hectares, with a total annual output of 17.332 million tons, accounting for 50% of the country's annual oil production. In 2018, my country imported 94.489 million tons of edible oilseeds, a year-on-year decrease of 7.4%, and imported edible vegetable oils reached 8.087 million tons, a year-on-year increase of 8.9%. According to experts from the Development Research Center of the State Council, my country's vegetable oil self-sufficiency rate will drop to 25% by 2025. Therefore, ...

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Problems solved by technology

[0004] What the present invention aims to solve is that the identification method of existing peanut varieties resistant to bacterial wilt disease has the defects of inaccurate results, uneven onset and poor repeatability, and further provides a peanut with better identification accuracy and repeatability. Field artificial inoculation identification method of cultivars resistant to bacterial wilt

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