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Application of arabidopsis transcription factor SRG1 gene in regulation and control of plant growth and development

A transcription factor and plant growth technology, applied in the field of genetic engineering, can solve the problems of plant size regulation that have not been reported

Pending Publication Date: 2022-06-07
XUZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the regulation of this gene in leaf development and plant size.

Method used

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  • Application of arabidopsis transcription factor SRG1 gene in regulation and control of plant growth and development
  • Application of arabidopsis transcription factor SRG1 gene in regulation and control of plant growth and development
  • Application of arabidopsis transcription factor SRG1 gene in regulation and control of plant growth and development

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Sterile culture and hypocotyl development of Arabidopsis thaliana

[0018] Seeds of Arabidopsis Col-0, srg1 and SRG-OX were taken, respectively, the seeds were placed in a 1.5 ml centrifuge tube, sterilized with 70% ethanol for 1 min, followed by 1% sodium hypochlorite (effective chlorine concentration of 5%) for 5-8 min, and finally washed four times with sterile water. Spread the seeds evenly on 1 / 2 MS solid medium.

[0019] After 2 days of dark treatment at 4 °C, the seeds were moved to a plant incubator, plants were cultured upright to 6 days of age under 10 h light: 16 h dark photoperiod conditions, followed by photographs of plant growth and measurement of plant root length ( Figure 1 )。

Embodiment 2

[0020] Example 2 Arabidopsis leaf size and number analysis

[0021] Seeds of Arabidopsis Col-0, srg1 and SRG-OX were sown to MS medium according to the sterile culture method in Example 1, treated in the dark at 4 °C for 2 days, and then placed in a plant incubator for horizontal culture. Plants are transplanted in soil (grass charcoal: vermiculite = 3:2) after 10 days in the incubator, and the plants are cultured under the same photoperiod conditions for the next experiment.

[0022] When the plant is 4 weeks old, take a picture of the fully extended rosette leaves of the plant and measure the leaf surface area at 6 weeks ( Figure 2 and Figure 3 )。 At the same time, the number of leaves of plants aged 4, 5 and 6 weeks was recorded respectively ( Figure 4 )。

Embodiment 3

[0023] Example 3 Arabidopsis plant type observation

[0024]Seeds of Arabidopsis Col-0, srg1 and SRG-OX were sown to MS medium according to the sterile culture method in Example 1, treated in the dark at 4 °C for 2 days, and then placed in a plant incubator for horizontal culture. After the plants were cultured in the incubator for 10 days, the plants were transplanted in the soil (grass charcoal: vermiculite = 3:2), and the plants were cultivated to 7 weeks later, the plant height and plant type were photographed and observed ( Figure 5 )。

[0025] The above provided embodiment is the arabidopsis transcription factor SRG1 gene applied to plant leaf size, hypocotyl development and plant size research.

[0026] Although, the present invention has been described in detail with a general description and specific embodiments above, but on the basis of the present invention, it may be slightly modified or improved, which is obvious to those skilled in the art. Similarly, the constructi...

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Abstract

The arabidopsis transcription factor SRG1 gene is applied to regulation and control of plant growth and development. T-DNA of the transcription factor gene SRG1 is inserted into the mutant srg1 to promote plant hypocotyl development, leaf enlargement, plant type enlargement and the like; after overexpression of the transcription factor gene SRG1, hypocotyl development is inhibited, leaf surface area is reduced, and plant type is reduced, which indicates that the transcription factor SRG1 plays an important role in regulation and control of plant development. Therefore, the SRG1 can be used as a potential molecular breeding tool to improve the plant type and increase the plant yield. Homologous genes in crops may have the same or similar functions, so that the homologous genes have important significance in production. The invention discloses the application direction of the transcription factor SRG1 in plant variety improvement, and the transcription factor SRG1 has important value for cultivating large-leaf or high-biomass transgenic plants.

Description

Technical field [0001] The present invention belongs to the field of genetic engineering, specifically, relates to the application of the Arabidopsis transcription factor SRG1 gene in the cultivation of plant leaves and hypocotyl growth and development and plant size. Background [0002] As a populous country, China has a huge demand for crops such as grain and vegetables every year. The size of the leaves and plant size of plants is of great significance for food crops, especially leafy vegetables and leafy cash crops. The growth and development of plants and the formation of morphology is a very complex process, which is ultimately controlled by genes, which is the selective expression of genes and the process of post-expression modification. The discovery of new plant growth and development regulation genes is necessary to understand the mechanism of plant development and use them to artificially improve the variety resources of crops. [0003] As one of the model plants in pl...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82A01H5/12A01H6/20
CPCC07K14/415C12N15/8261Y02A40/146
Inventor 潘巧娜崔北米加利·约翰·洛克
Owner XUZHOU NORMAL UNIVERSITY
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