Preparation method of soluble microneedle patch constructed based on graphene quantum dots and used for treating bacterial keratitis

Abstract

The invention relates to a preparation method of a soluble microneedle patch constructed based on graphene quantum dots for treating bacterial keratitis, which comprises the following steps: S1, taking 20-30 [mu] L of imidazole modified graphene quantum dots (IMZ-GQDs) solution (30 mg / mL), dropwise adding on the surface of a PDMS template, and placing in a vacuum drying oven; s2, taking out, scraping redundant solution on the surface, and performing vacuum drying again; s3, taking about 25 microliters of PVA / PVP mixed solution, dropwise adding the PVA / PVP mixed solution on the surface of the PDMS template, and placing the PDMS template in a vacuum drying oven; s4, taking out, and scraping redundant solution and bubbles on the surface; and S5, drying at room temperature for 24 hours, demolding, and storing in a dryer. The IMZ-GQDs MNs disclosed by the invention have the beneficial effects that the IMZ-GQDs MNs combines an antibacterial nano material with a microneedle technology, is used for safe, efficient and controllable eye administration, can effectively break through an ocular surface anatomical barrier, and can simultaneously dissolve and release IMZ-GQDs to destroy the integrity of a bacterial inner membrane and reduce the membrane potential to finally cause bacterial death, so that the purpose of treating bacterial keratitis is achieved.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a preparation method of a soluble microneedle patch constructed based on graphene quantum dots for treating bacterial keratitis. Background technique [0002] Graphene quantum dots (GQDs) have the advantages of high photostability, good solubility, easy modification and low toxicity. May not be suitable for sensitive tissues such as the ocular surface. [0003] Positively charged nano-antibacterial materials have attracted much attention in recent years. As one of the most common five-membered heterocyclic compounds in nature, imidazole (IMZ) is a component of histidine and participates in various enzymatic reactions in vivo. The imidazole ring can be converted into a positively charged cation through an alkylation reaction, and has good stability and safety, and is widely used in the pharmaceutical field for the treatment of microbial infections, cancer, Alzheimer's disease, ...

AI Technical Summary

Problems solved by technology

[0004] In addition, there are still challenges in the effective drug delivery of nanomaterials to the eye. It is difficult for the form of eye drops / ointments to break through the anatomical barrier to reach an effective therapeutic concentration, and increasing the application concentration may increase cytotoxicity; the drug delivery system implanted in the eye can achieve Controlled release of drug, but surgical intervention is required, risky

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