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Culture method for promoting microalgae fucoxanthin accumulation and lipid synthesis

A technology of microalgae fucoxanthin and a culture method is applied in the culture field of promoting microalgae fucoxanthin accumulation and lipid synthesis, and achieves the effects of promoting accumulation, strong practicability and improving antioxidant capacity

Pending Publication Date: 2022-05-31
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the cultivation process of Phaeodactylum tricornutum, the effect of adding a certain amount of L-ascorbic acid on its cell metabolism has not been reported so far.

Method used

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  • Culture method for promoting microalgae fucoxanthin accumulation and lipid synthesis
  • Culture method for promoting microalgae fucoxanthin accumulation and lipid synthesis
  • Culture method for promoting microalgae fucoxanthin accumulation and lipid synthesis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: The effect of adding a certain amount of L-ascorbic acid on the oil content of marine microalgae in the microalgae culture medium

[0025] (1) Preparation of algae seed liquid: culture a certain amount of microalgae at a temperature of 21°C, a light intensity of 150 lux, and a light-to-dark ratio of 12h / 12h to the logarithmic growth phase to obtain algae liquid. The microalgae are made of marine silicon Alga Phaeodactylum tricornutum.

[0026] (2) Medium configuration: Each 1L of the medium contains the following components: 1 mg ~ 100 mg NaNO 3 , 1 mg ~50 mg NaH 2 PO 4 2H 2 O, 1 mg ~ 50 mg Na 2 EDTA, 1 mg ~50 mg FeCl 3 ·6H 2 O, 0.01 μg ~ 1 mg CuSO 4 ·5H 2 O, 0.01 μg ~1mg ZnSO 4 ·7H 2 O, 0.01 μg ~1mg CoCl 2 ·6H 2 O, 0.01 μg ~1 mg MnCl 2 · 4H 2 O, 0.0001 μg ~1mg Na 2 MoO 4 · 2H 2 O, 0.0001 μg ~1mg vitamin B12, 0.01 μg ~10mg vitamin B1 and 0.0001 μg ~1mg vitamin H. The initially prepared medium is the medium prepared when the microalgae have...

Embodiment 2

[0030] Embodiment 2: The effect of adding a certain amount of L-ascorbic acid on the lipid components of marine microalgae in the microalgae culture medium

[0031] (1) Preparation of algae seed solution: refer to Example 1.

[0032] (2) Culture medium configuration: refer to Example 1.

[0033] (3) Drug induction: refer to Example 1.

[0034] (4) Evaluation index: analysis of fatty acid composition of microalgae.

[0035] After the microalgae were cultured to the plateau stage, 200 mL of the culture solution was taken from each group, centrifuged at 4400 rpm for 10 min at 4 °C, freeze-dried immediately, and weighed. Transfer the freeze-dried algae pellets to 10 mL test tubes and add 5 mL of KOH-CH to each test tube 3 OH solution and sonicate the cells in an ice bath. Seal the test tube, treat it under the nitrogen flow of a nitrogen blower for 1 min, and then incubate at 75° C. for 10 min. After cooling to room temperature, transfer the solution supernatant to a 50 mL tu...

Embodiment 3

[0041] Example 3: Effect of adding a certain amount of L-ascorbic acid on the content of marine microalgae fucoxanthin in the microalgae culture medium

[0042] (1) Preparation of algae seed solution: refer to Example 1.

[0043] (2) Culture medium configuration: refer to Example 1.

[0044] (3) Drug induction: refer to Example 1.

[0045] (4) Evaluation index: analysis of microalgae fucoxanthin content.

[0046] Take a certain amount of fucoxanthin standard, and use absolute ethanol to configure, in A 445 The absorbance was measured under the conditions, and the obtained data were used to draw the standard curve (R 2 ≥0.999).

[0047] Take a certain quality of algae powder in a centrifuge tube, add 600-1000 μL of absolute ethanol under the condition of avoiding light, vortex, heat in a constant temperature water bath at 75°C for 2 hours, and vortex every 15 minutes during this period. After cooling to room temperature, centrifuge at 8000rpm for 5min, and take a certain a...

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Abstract

The invention discloses a culture method for promoting accumulation of microalgae fucoxanthin and lipid synthesis, which comprises the following steps: inoculating microalgae into a culture medium for culture, and adding 150-350mg / L of L-ascorbic acid into the culture medium. According to the culture method disclosed by the invention, the fucoxanthin content of the phaeodactylum tricornutum can be increased, and meanwhile, the lipid content of the phaeodactylum tricornutum, especially the content of eicosapentaenoic acid (EPA), is increased.

Description

technical field [0001] The invention relates to the technical field of cultivation of algal organisms, in particular to a cultivation method for promoting the accumulation of microalgae fucoxanthin and lipid synthesis. Background technique [0002] Diatoms are an important part of the primary productivity of the ocean, and their influence on the earth's ecological environment and carbon cycle cannot be underestimated (Armbrust, 2009; Malviya et al., 2016). As a common marine diatom, Phaeodactylum tricornutum has become one of the hot research organisms for biological carbon sequestration because of its high content of oil and fucoxanthin. [0003] Fucoxanthin is a unique active ingredient in brown algae, such as Phaeodactylum tricornutum ( Phaeodactylum tricornutum ), Japanese sugar kelp ( Saccharina japonica ),Wakame( Undaria pinnatifida ) etc. (Peng et al., 2011). The special chemical structure of fucoxanthin makes it not only play a role in plant photoprotection, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/38C12P17/02C12P7/6427C12P7/649A23L33/10C12R1/89
CPCC12N1/12C12N1/38C12P17/02C12P7/6427C12P7/649A23L33/10Y02E50/10
Inventor 张惠莹殷炜铧刘建秦源陆瑶胡育川董广辉谢璇
Owner FUJIAN AGRI & FORESTRY UNIV
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