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Application of Nnufs1 gene in treatment of heart failure after myocardial infarction and related product

A gene and product technology, applied in the field of biology and new medicine, can solve the problems of unclear potential mechanism of action, and achieve the effect of alleviating heart failure after myocardial infarction, reducing cell apoptosis, and reducing myocardial fibrosis

Active Publication Date: 2022-05-17
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the role of Ndufs1 in heart disease and its underlying mechanisms remain unclear

Method used

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  • Application of Nnufs1 gene in treatment of heart failure after myocardial infarction and related product
  • Application of Nnufs1 gene in treatment of heart failure after myocardial infarction and related product
  • Application of Nnufs1 gene in treatment of heart failure after myocardial infarction and related product

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Experimental program
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Effect test

Embodiment Construction

[0029] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0030] One, such as figure 1 As shown, the present invention provides an AAV9-based Ndufs1 overexpression vector, which is used for the treatment of heart failure after myocardial infarction, and its construction method is as follows:

[0031] 1. Gene basic information

[0032] Gene name: Ndufs1 (NM_145518-3flag)

[0033] Gene ID: 227197

[0034] Species: Mouse

[0035] CDS length: 2184bp

[0036] The base sequence of the CDS region is shown in SEQ ID NO:1.

[0037] 2. Digestion of vector

[0038] 2.1 Carrier information:

[0039] Select the appropriate carrier according to the base length of Ndufs1 gene, which carries the heart-specific promoter cTNT. The information of the carrier used in this experiment is as follows:

[0040] Carrier name: GV618

[0041] Element sequence: cTNTp-MCS-SV40 PolyA

[0042] Cloning site: AgeI / HindIII

...

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Abstract

The invention discloses application of a Nnufs1 gene in treatment of heart failure after myocardial infarction and related products, up-regulation of the Nnufs1 can significantly reduce apoptosis and mitochondrial respiratory dysfunction caused by hypoxia, which is extremely beneficial to improvement of myocardial cell functions and reduction of cell death. The NDufs1 overexpression vector based on AAV can significantly improve myocardial fibrosis and cardiac dysfunction after myocardial infarction. Therefore, aiming at the new target Nnufs1, AAV-based gene therapy is expected to become an effective means for treating or preventing heart failure after myocardial infarction.

Description

technical field [0001] The invention belongs to the technical field of biology and new medicine, in particular to an application of Ndufs1 gene in treating heart failure after myocardial infarction and related products. Background technique [0002] Globally, cardiovascular disease is the most common cause of death. Heart failure is the end-stage manifestation of most cardiovascular diseases, its long-term prognosis is poor, and its 5-year mortality rate exceeds that of some common cancers. Myocardial infarction has been the most common cause of heart failure for the past 20 years. During myocardial infarction, the sudden interruption or reduction of blood supply results in the death of cardiomyocytes or the dysfunction of remaining surviving cardiomyocytes. Apoptosis and myocardial fibrosis are important pathological features of adverse myocardial remodeling after myocardial infarction. Although current advances in interventional and drug strategies have brought about be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/52C12N15/864C12N5/10A61K48/00A61K38/43A61P9/04A61P9/10
CPCC12N9/00C12N15/86C12N5/0657A61K48/0058A61K48/005A61K38/43A61P9/04A61P9/10C12N2750/14143C12N2800/107C12N2830/008C12N2510/00Y02A50/30
Inventor 亓秉超李妍张明明胡建强胡朗任高彤彭庭伟刘铭传郭栋
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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