Microsiphum avenae repellent and application thereof
A long tube aphid, repellent technology, applied in animal repellent, application, pest repellent, etc., can solve the problem of excessive pesticide residues, restricting the sustainable development of chrysanthemum industry, affecting the quality and safety of chrysanthemum products and the ecological environment Safety and other issues, to achieve the effect of easy promotion, promotion of sustainable development, and obvious repelling effect
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Embodiment 1
[0024] Example 1 Behavior selection test of Aphid chrysanthemum to 4 kinds of volatile compounds
[0025] 1. Material method
[0026] 1.1 Breeding and collection of Aphis chrysanthemum
[0027] Aphid chrysanthemum was collected from the experimental greenhouse of the National Flower Engineering Technology Research Center of Beijing Forestry University. Before the experiment, the 4th instar wingless nymphs of Aphis chrysanthemum were collected, inoculated on the aphid-susceptible chrysanthemum variety 'Jinglin Qiuge', and raised in gauze bags. During the Y-tube behavior selection test, wingless adult aphids were randomly collected from gauze bags for behavioral tests.
[0028] 1.2 Preparation of 4 kinds of volatile chemical standards
[0029] Among the 4 volatile matter standards, β-ocimene (CAS number: 13877-91-3; Sigma product number: W353901) and cis-4-thujyl alcohol (CAS number: 546-79-2; Sigma product number: 96573) was purchased from Sigma Reagent China Company; L-bor...
Embodiment 2
[0039] Example 2 Interference of cis-4-thujol and α-humulene on feeding behavior of Aphid chrysanthemum
[0040] According to the results of Example 1, both 0.1% cis-4-thujyl alcohol and 1% α-humulene can significantly repel adults of Aphis chrysanthemum. This example further verifies the interference effect of these two compounds on Aphis chrysanthemi in the actual feeding state.
[0041] 1. Material method
[0042] When the cutting seedlings of the chrysanthemum 'Jinglin Qiuge' grow to 8-10 leaves, inoculate 5 adult aphids of Chrysanthemum chrysanthemum, and bag them to reproduce freely. After 10 days, a certain number of aphids proliferated on the inoculated chrysanthemums, and they were all in a normal feeding state. At this time, using a 10 μl pipette gun, pipette 10 μl of solvent n-hexane (as a control), 0.1% cis-4-thujyl alcohol and 1% α-humulene solution respectively, and place them on the 2- Release around 3cm. A video camera was used to record the behavioral resp...
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