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Bacillus coagulans and application thereof in corn soaking

A Bacillus coagulans and corn soaking technology, applied in the direction of bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of high usage, long soaking time, high energy consumption, etc., and achieve reduced sulfur dioxide consumption and soaking effect Good, high starch yield effect

Pending Publication Date: 2022-05-13
JILIN COFCO BIOCHEM +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is in order to overcome SO in the corn steeping process that prior art exists 2 Problems such as high usage, long soaking time, and high energy consumption provide a strain of Bacillus coagulans and its application in corn soaking. When soaking corn with bacteria agent, it can shorten the soaking time and reduce SO 2 Usage amount, at the same time, compared with the corn soaking process of auxiliary soaking using enzyme preparations or bacterial agents in the prior art, the method provided by the invention is simpler to operate and easier to industrialize.

Method used

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  • Bacillus coagulans and application thereof in corn soaking
  • Bacillus coagulans and application thereof in corn soaking
  • Bacillus coagulans and application thereof in corn soaking

Examples

Experimental program
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Effect test

Embodiment 1

[0051] This example is used to illustrate the isolation, purification and preservation of Bacillus coagulans CGMCC No. 23993 provided by the present invention.

[0052] (1) Strain isolation and purification

[0053] The inventor of the present invention isolated and obtained a strain of Bacillus coagulans CGMCC No.23993 from the corn soaking liquid used in the countercurrent soaking process in the corn starch production line of COFCO Biochemical Energy (Yum) Co., Ltd. The specific separation and purification process is as follows:

[0054] Corn steep liquor dry powder liquid medium is used as the enrichment medium, and its composition is: glucose 20g / L, beef extract 10g / L, peptone 10g / L, yeast extract 5g / L, triammonium citrate 2g / L, sodium acetate 5g / L, dipotassium hydrogen phosphate 2g / L, magnesium sulfate 0.2g / L, manganese sulfate 0.05g / L.

[0055] Take COFCO Biochemical Energy (Yumshu) Co., Ltd., 0.5mL of corn soaking solution from the countercurrent soaking process of c...

Embodiment 2

[0060] This example is used to illustrate the improvement of the corn soaking effect of Bacillus coagulans CGMCC No.23993 provided by the present invention.

[0061] Bacillus coagulans DUT21 was cultured with corn steep liquor dry powder medium, and the specific culture conditions included: 200rpm shaker culture at a temperature of 50±2°C for 24 hours to obtain DUT21 culture medium for use. After testing the OD of the DUT21 culture medium 600 The value is about 6.1±0.7 (equivalent to the effective number of viable bacteria of DUT21 is about 6.08×10 8 CFU / mL).

[0062] (1) Effect of Bacillus coagulans DUT21 inoculation amount on corn soaking effect

[0063] The inoculation amount of the DUT21 culture solution is 4 volume%, 6 volume%, 8 volume%, 10 volume%, 12 volume%, and the total volume is 150mL (remainder is filled with sterile water). A certain amount of SO was introduced into the soaking solution 2 , so that SO in the soaking solution 2 The concentration is 1000ppm. ...

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Abstract

The invention relates to the technical field of corn deep processing, and particularly discloses bacillus coagulans and application thereof in corn soaking, and the preservation number of the bacillus coagulans is CGMCC (China General Microbiological Culture Collection Center) No.23993. The bacillus coagulans provided by the invention has the characteristics of acid resistance, high temperature resistance and the like, and when the bacillus coagulans is used for soaking corn, the yield of corn starch can be remarkably increased, the soaking time can be shortened, and the usage amount of SO2 can be reduced. Besides, when the bacillus coagulans provided by the invention is used for soaking the corn, corn kernels do not need to be crushed, and the corn can be soaked by adopting a one-step method, so that the process is simplified, and the bacillus coagulans is more suitable for industrial production and application.

Description

technical field [0001] The invention relates to the technical field of corn deep processing, in particular to a strain of bacillus coagulans and its application in corn soaking. Background technique [0002] The production of corn starch is mainly divided into dry method and wet method, among which the wet method is a kind of starch preparation process widely used at present. In the wet process, after soaking the corn kernels, they are coarsely and finely ground to separate the germ, fiber and protein, and finally obtain high-purity cornstarch. Soaking is the first and most important process in the wet process of corn starch production. The effect of soaking has a significant impact on the yield of corn starch. [0003] The purpose of soaking is to soften the corn kernels, destroy or weaken the binding force between protein and starch granules, and free the starch. At present, the conventional corn soaking process usually needs to add a higher concentration of SO to the so...

Claims

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Application Information

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IPC IPC(8): C12N1/20C08B30/02C12R1/07
CPCC12N1/20C08B30/02
Inventor 佟毅孙亚琴夏文静修志龙赵雪松李义金渭武王小艳
Owner JILIN COFCO BIOCHEM
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