Large-scale preparation method of natural gynogenesis bighead carp derived from distant hybridization

Abstract

The invention discloses a large-scale preparation method of natural gynogenetic bighead carp derived from distant hybridization, which comprises the following steps: by taking bighead carp as a female parent and xenocypris davidi as a male parent, injecting a mixed oxytocic of luteinizing hormone releasing hormone analogue, chorionic gonadotropin and pituitary into the female parent in a breeding season; then artificially injecting a mixed oxytocin of luteinizing hormone releasing hormone analogue and chorionic gonadotropin into the male parent fish for artificial spawning induction; and after the injection is finished and the parent fish is in an oestrus, carrying out artificial insemination, hatching in running water, feeding, detecting and screening to obtain the natural gynogenesis bighead carp. According to the method, the gynogenesis offspring can be obtained without technical treatment such as sperm inactivation and ovum doubling, and the method has the obvious advantages that the fertilization rate is high, the hatching rate is high, a large number of natural gynogenesis bighead carps with amphoteric fertility can be obtained, and the breeding workload is greatly reduced; by means of the method, the natural gynogenesis bighead carps which are high in growth speed, strong in disease resistance, good in meat quality and omnivorous and can be fertile in two sexes are obtained.

Description

technical field [0001] The invention belongs to the technical field of fish distant hybrid breeding, in particular to a large-scale preparation method of natural gynogenetic bighead carp derived from distant hybrid. Background technique [0002] Distant hybridization refers to the hybridization between different species or genus and above species, which can integrate the genomes of different species, so that the hybrid offspring will produce changes at the genome level. Hybrid offspring with altered genotype and phenotype can be produced. Distant hybridization can also produce gynogenetic offspring, which is an important way to produce natural gynogenetic offspring. Moreover, the offspring of natural gynogenesis produced by this method will have heterosperm hybridization effect, so as to obtain offspring with good performance in meat quality, disease resistance, stress resistance and growth speed, etc. Sexually fertile natural gynogenetic offspring provide a unique germpla...

AI Technical Summary

Problems solved by technology

However, since bighead carp was mainly zooplankton in the past, but limited by the lack of natural food resources in the water body, the output and commodity specifications of bighead carp are far from meeting the growing market demand for commercial bighead carp

[0004] Moreover, in the previous research technology, the operation process to obtain natural gynogenetic offspring is cumbersome, and the selection requirements for sperm parent, sperm inactivation method and chromosome doubling method are very strict, and the obtained gynogenetic offspring are all female offspring

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