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Blood type serological analysis card comprising blood type card and anti-human globulin card and preparation method thereof

An anti-human globulin and analysis card technology, applied in the field of blood group serology analysis card and its preparation, can solve the problems of inconvenient storage and transportation, many influencing factors, variable particle size, etc. The effect of high simulation degree and high particle size uniformity

Pending Publication Date: 2022-05-10
胡佚凡
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] (1) The mechanical strength is low, and it is easy to deform during high-speed centrifugation. Therefore, to ensure the stability of the pore size of the cell sieve, the proper centrifugation effect can only be achieved by prolonging the centrifugation time at low speed, thereby reducing the detection speed;
[0008] (2) Low density, poor self-lubricating property and weak coagulation. The bumps or inversions during transportation can cause the redistribution of gel particles, which eventually leads to a larger pore size of the cell sieve, which must be re-centrifuged before it can be used. workload;
[0009] (3) Low temperature resistance, the swelling rate of dextran gel will change with the change of ambient temperature, causing the particle size of dextran gel particles to change, which directly leads to the large and small pore size of the cell sieve, In order to ensure stable quality, it must be transported or stored at 2-25°C, which brings inconvenience to storage and transportation;
[0010] (4) The uniformity of particle size is poor, the technical requirements for producing dextran gel are high, and there are many influencing factors, which lead to large differences in the size of dextran gel particles, which affect the stability of the experiment
[0011] (5) The human body’s pH simulation is not good, and the stability of dextran gel particles is easily affected by pH changes. Therefore, the pH of the dextran gel particle system currently used in blood type serological analysis is between 6.5 and 7.0 , significantly lower than the 7.35-7.45 of the normal human body
Therefore, the antigen-antibody response in the dextran gel particle system cannot fully represent the antigen-antibody response in the in vivo environment
[0012] (6) The production process of dextran gel particles used for cell separation is complicated. Up to now, this technology has only been mastered by a few countries in Europe and the United States. Dependency and high price

Method used

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  • Blood type serological analysis card comprising blood type card and anti-human globulin card and preparation method thereof
  • Blood type serological analysis card comprising blood type card and anti-human globulin card and preparation method thereof
  • Blood type serological analysis card comprising blood type card and anti-human globulin card and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1: Preparation of blood type serological analysis card of the present invention

[0059] 1. Preparation of blood type card (for ABO and Rh D blood type detection)

[0060] The blood type card is composed of 6 microcolumns, the first 3 of which are sequentially filled with a mixture of IgM anti-A antibody and polymethylmethacrylate (polymethylmethacrylate, PMMA) microspheres, and a mixture of IgM anti-B antibody and PMMA microspheres. , and the mixture of IgM anti-D antibody and PMMA microspheres, and the latter three are evenly distributed with PMMA microsphere suspension. The specific implementation includes the following steps:

[0061] (1) Preparation of PMMA microsphere suspension matrix

[0062] Use distilled water to dissolve the following solutes in their respective proportions:

[0063]

[0064] Adjust the pH to 7.35-7.45 and mix well;

[0065](2) Screening of PMMA microspheres with a diameter of 80-150 μm

[0066] Use 180 mesh and 100 mesh sc...

Embodiment 2

[0103] Example 2: Verification of the application of the novel anti-human globulin card in high-speed centrifugation of the present invention

[0104] The new anti-human globulin card was prepared by the method of Example 1. The traditional Sephadex anti-human globulin card was purchased from the market, and all the Sephadex anti-human globulin cards used were the same.

[0105] (1) Irregular antibody screening negative and positive tests

[0106] Normal centrifugation: take a Sephadex anti-human globulin card as the control group, and add irregular antibody screening positive plasma and corresponding irregular antibody screening cells confirmed by the Coombs test to the first two wells as positive controls , the latter two wells were used as negative controls to add irregular antibody screening negative plasma and corresponding irregular antibody screening cells confirmed by the Coombs test, and after incubation at 37°C for 15 minutes, centrifugation was carried out using the...

Embodiment 3

[0111] Embodiment 3: PMMA microsphere sealing effect verification

[0112] The new anti-human globulin card was prepared by the method of Example 1. The traditional Sephadex anti-human globulin card was purchased from the market, and all the Sephadex anti-human globulin cards used were the same.

[0113] (1) Irregular antibody screening negative and positive tests

[0114] Take a dextran gel anti-human globulin card as the control group, add irregular antibody screening positive plasma and corresponding irregular antibody screening cells confirmed by the Coombs test to the former hole as a positive control, As a negative control, add irregular antibody screening negative plasma and corresponding irregular antibody screening cells confirmed by the Coombs test, incubate at 37°C for 15 minutes, and then centrifuge under the conditions recommended by the manufacturer (100g, centrifuge for 9 minutes);

[0115] Take a new type of anti-human globulin card prepared by blocked PMMA mi...

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Abstract

The invention relates to the technical field of in-vitro diagnosis, and particularly discloses a blood type serological analysis card comprising a blood type card and an anti-human globulin card and a preparation method of the blood type serological analysis card. According to the blood type serology analysis card, efficient analysis of blood type serology is achieved by means of the mechanism that PMMA microspheres with a certain diameter form a molecular sieve, and meanwhile the blood type serology analysis card is high in filler particle size uniformity, good in temperature resistance effect, small in result interpretation interference, high in human body environment simulation degree, low in external technology dependency and low in price and has good application prospects. And a guarantee is provided for blood type identification, irregular antibody screening and cross blood matching tests.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis, in particular to a blood group serological analysis card including a blood group card and an antihuman globulin card and a preparation method thereof. Background technique [0002] Blood transfusion is one of the essential clinical treatment methods, mainly used to improve hypoxia and correct blood coagulation. Safe and effective blood transfusion can quickly alleviate the condition and buy time for further treatment. However, if not handled properly, blood transfusion can also cause a variety of adverse reactions, aggravate the patient's condition and even threaten life. Therefore, before blood transfusion, all patients need to undergo blood group serological analysis, including blood type identification, cross-matching and irregular antibody screening, to ensure that the red blood cells transfused into the recipient and the recipient's own red blood cells are not damaged. The specif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/80G01N33/68G01N33/577
CPCG01N33/80G01N33/6854G01N33/577
Inventor 胡佚凡
Owner 胡佚凡
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