Method for manufacturing paraffin section by using embedding machine

Abstract

The invention belongs to the technical field of slice preparation, and discloses a method for preparing paraffin slices by using an embedding machine. Taking materials, fixing, cutting, putting into a bottle, and fixing for 30 hours; dehydrating and treating with a transparent agent: dehydrating for 3 hours, and treating with the transparent agent for 1.5 hours; the dyeing adopts sarranine and fast green counter dyeing; paraffin refining, wherein paraffin soaking is conducted in a 75-DEG C constant-temperature box; embedding by adopting a semi-automatic embedding machine, putting the material into an embedding box, arranging the position of the material, putting the embedding box on ice cubes made by an ice crusher, and rapidly cooling; trimming blocks, slicing, bonding and dewaxing; and sheet sealing: carrying out wet sealing and natural air drying. According to the method, the internal tissue structure of the plant is defined, key links such as material taking and fixing, dehydration and transparent agent treatment, wax melting, wax soaking and embedding, slicing, slice bonding, dyeing and slice preparation are optimized, time is saved, and the slice preparation efficiency and the success rate are improved.

Description

technical field [0001] The invention belongs to the technical field of slice making, and in particular relates to a method for making paraffin slices by using an embedding machine. Background technique [0002] Because of its advantages of economy, convenience, applicability, simplicity, easy operation, low equipment requirements, and long-term storage of materials, paraffin section technology has become the most widely used in routine histological and pathological techniques. It plays a very important role in the field of scientific and technological research. However, there are often many problems in the process of making paraffin sections, such as non-banding, bending, cracks, shrinkage, non-sticking, falling off, and uneven thickness. The methods adopted in each stage are different. In the actual operation process, the experimental instructions or literature materials cannot be copied, but repeated exploration is required to produce an ideal film. [0003] In summary, ...

AI Technical Summary

Problems solved by technology

[0004] (1) In the process of making paraffin slices in the prior art, there are often slices that are not banded, bent, cracked, shrunk, non-stick, falling off, and uneven in thickness

[0005] (2) In the prior art, different methods are adopted in different crops, different organs of the same crop, and different developmental stages of the same organ, which brings huge workload and reduces work efficiency