Application of GT1/HB13 gene in regulation and control of non-degeneration and lodging resistance of inferior flower of corn ear

Abstract

The invention discloses application of GT1 and HB13 genes in regulation and control of non-degeneration and lodging resistance of corn female ear lower flowers. The method comprises the following steps: carrying out gene editing on two targets, namely GT1 and HB13 in corn by utilizing a CRISPR / Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats / CRISPR associated 9) technology; the result shows that compared with a wild type and two single-process materials, the phenotype of the gt1 / hb13 double-mutant plant is more violent, the tiller number is obviously increased, the root system is more developed, the cluster number is increased, new female ears grow on ear stems of main ears, multi-ear property is shown, axillary meristem at the lower part is developed into female ears without inhibition, lower flowers in the female ears are not degraded, and the yield of the gt1 / hb13 double-mutant plant is increased. After the GT1 and HB13 genes are knocked out, the heart skin of the inferior flowers continuously develops to form filaments, some inferior flowers can also form two filaments, the phenomenon of multiple filaments is shown, the inferior flowers continuously develop to form extra small grains in the later period, and it is shown that after the GT1 and HB13 genes are knocked out, a corn mutant plant can show strong female spike inferior flower non-degeneration phenotype and lodging resistance.

Description

technical field [0001] The invention relates to the technical field of maize genetics and breeding, and more specifically relates to the application of GT1 and HB13 genes in regulating the non-degeneration and lodging resistance of lower flowers of maize ears. Background technique [0002] Corn (Latin scientific name: Zea mays L.) is the crop with the highest economic value in the world. It is an important resource for both food, feed and bioenergy, and is particularly important for ensuring food security and economic development in my country. The inflorescence is the reproductive organ of the plant and the carrier of flowers and fruits. Its structure and morphological establishment directly affect the number of flowers, fruit quantity, size, and breeding system of the plant, and then affect the yield of the crop. Early studies believed that maize sex differentiation involves fine regulation of a variety of endogenous hormones, sex differentiation-related genes, external en...

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Problems solved by technology

[0004] Chinese patent CN111909936A discloses that after the HD-ZIP I subfamily gene Grassy tillers1 (GT1) mutation in maize, the carpels in the tassel florets of the plant do not degenerate and grow filaments, but the number of filaments is not much, indicating that the ear floret carpels Developmental abnormalities, and the impact of simultaneous mutations of GT1 and its homologous gene HB13 on maize traits has not been reported in the prior art

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