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Arthrobacter capable of degrading erucyl amide as well as acquisition method, culture method and application of arthrobacter

A technology for obtaining erucamide, which is applied in the direction of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of inability to process erucamide, high cost, secondary pollution, etc., and achieve rapid The effect of degrading COD, huge technical prospects, and large technical support

Pending Publication Date: 2022-05-06
恒臻无锡生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the deficiencies of the above-mentioned prior art, the present invention provides Arthrobacter that can degrade erucamide and its acquisition method, culture method and application. Erucamide often needs to introduce other chemical substances, which is not only easy to cause secondary pollution, but also high in cost, and there are almost no suitable strains for the degradation of erucamide in the biological method.

Method used

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  • Arthrobacter capable of degrading erucyl amide as well as acquisition method, culture method and application of arthrobacter
  • Arthrobacter capable of degrading erucyl amide as well as acquisition method, culture method and application of arthrobacter
  • Arthrobacter capable of degrading erucyl amide as well as acquisition method, culture method and application of arthrobacter

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Effect test

Embodiment 1

[0030] This embodiment provides a method for obtaining Arthrobacter that can degrade erucamide, and the specific technical scheme is as follows:

[0031] 1. Screening and identification of strains

[0032] (1) Isolation and purification of strains

[0033]The activated sludge in the biochemical pool of a printing and dyeing wastewater treatment plant was used as the separation mud. The activated sludge is added into the inorganic salt liquid medium containing erucamide according to the ratio of 10% to carry out enrichment culture for 2-7 days. Inorganic salt liquid medium containing erucamide includes NH4Cl 0.38g / L, NaCl 5g / L, K2HPO4 0.5g / L, KH2PO4 0.5g / L, MgSO4·7H2O 0.2g / L, CaCl2 0.01g / L, FeSO4· 7H2O0.001g / L and erucamide 0.3-0.9g / L.

[0034] On the inorganic salt solid medium containing erucamide, the enriched bacterial solution was separated and purified by dilution coating method and streaking method, and 3 purified strains were obtained. Inorganic salt solid medium is...

Embodiment 2

[0046] 1. Growth characteristics of strains

[0047] (1) Adaptability of strains to initial pH

[0048] Strain activation: inoculate the strain zs1 in the inorganic salt medium containing erucamide, and culture it with shaking at 30°C and 160r / min for 48-52h.

[0049] Adjust the initial pH value of the inorganic salt medium with erucamide as the carbon source to 3, 5, 7, 9 and 11 respectively, and inoculate the above-mentioned activated bacteria into the medium according to the ratio of 1%, and put it in a shaker (30°C, 160r / min), after culturing for 48 hours, measure the cell concentration and the degradation rate of erucamide. The result is as Figure 4 It was shown that when the initial pH was too high or too low, it was not conducive to the growth of the strain. The suitable pH range for the growth of the strain zs1 was 5.0-7.0, and the optimum pH was 7.0.

[0050] (2) Effect of erucamide concentration on the strain

[0051] On the basis of inorganic salt medium with e...

Embodiment 3

[0061] This embodiment provides the application of Arthrobacter that can degrade erucamide, and the specific technical scheme is as follows:

[0062] The effluent COD of a printing and dyeing wastewater treatment plant exceeded the standard, and the effluent water samples were detected and analyzed by GC-MS, and it was found that it mainly contained erucamide, such as Figure 9As shown, the peak at 48.64 was analyzed as erucamide, and the concentration was 0.083g / L. The erucamide-degradable Arthrobacter in Example 2 was inoculated at the front end of the aerobic tank of the sewage treatment plant at a ratio of 1‰. After 3 days, the COD in the effluent had dropped below the standard 50mg / L. Samples were taken to detect erucamide in the effluent from the aerobic pool, and it was found that the concentration of erucamide had dropped to 0.029g / L, and the degradation rate had reached 65%. It shows that when the erucamide-degrading bacteria are used to treat large-scale printing an...

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Abstract

The invention belongs to the field of microbial technology and environmental protection, and particularly relates to arthrobacter capable of degrading erucyl amide as well as an acquisition method, a culture method and application of the arthrobacter. The arthrobacter capable of degrading erucyl amide, provided by the invention, is the only strain capable of degrading erucyl amide from a biochemical pool of a printing and dyeing wastewater and sewage treatment plant at present, so that the arthrobacter can be applied to the field of printing and dyeing wastewater treatment, the degradation rate of erucyl amide is greater than 55%, no pollution is caused to the environment, and the effect of rapidly degrading COD is achieved; the method provides great technical support for biological treatment of erucyl amide, brings huge technical prospects for printing and dyeing sewage treatment, and has great significance in the printing and dyeing sewage industry.

Description

technical field [0001] The invention belongs to the technical field of microorganisms and environmental protection, and in particular relates to an Arthrobacter capable of degrading erucamide, an acquisition method, a culture method and application thereof. Background technique [0002] Erucamide, molecular formula C 22 h 43 NO, one of the most important uses is as antistatic agent, slip agent, release agent and anti-adhesive agent. It is widely used in plastic film products, ink and rubber, and erucamide is widely used in high-grade lubricating oil, dye dispersant, papermaking and textile industries. In the printing and dyeing textile industry, erucamide is often used in the pretreatment process of yarns and knitted fabrics to make the fabric soft and wrinkle-resistant, and the general addition amount is 0.05%-0.2%. When analyzing the characteristic pollutants of wastewater from the printing and dyeing textile industry by GC-MS, it is found that the production process of...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C02F3/34C12R1/06
CPCC12N1/02C12N1/20C02F3/34
Inventor 邓敬轩单晓红邱勋
Owner 恒臻无锡生物科技有限公司
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