Application of isoliensinine in preparation of medicine for treating leukemia

A technology of isoliensinine and leukemia, applied in the field of application of isoliensinine in the preparation of medicines for treating leukemia

Pending Publication Date: 2022-05-06
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no studies have shown whether isoliensinine has antitumor effects on hematological malignancies

Method used

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  • Application of isoliensinine in preparation of medicine for treating leukemia
  • Application of isoliensinine in preparation of medicine for treating leukemia
  • Application of isoliensinine in preparation of medicine for treating leukemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Effects of isoliensinine on cell viability of leukemia cell lines

[0025] 1.1. Experimental materials

[0026] 1.1.1 Reagents and materials

[0027] ① compound

[0028] Isoliensinine (C 37 h 42 N 2 o 6 , Molecular weight: 610.74) provided by the Institute of Medicinal Fungi and Traditional Chinese Medicine Biotechnology, Nanjing University of Traditional Chinese Medicine, light yellow powder, purity > 95%. Before use, the compound powder was prepared into a stock solution with a concentration of 0.05M with dimethyl sulfoxide, and stored at -80°C. Dilute to the desired concentration with the corresponding medium for cell culture.

[0029] ② cell line

[0030] Human AML cell lines include THP1, ME1, and MV411 cells, human CML cell lines include K562 cells, human T cell malignant tumor cells include Jurkat, Hut102, and Molt4 cells, and human DLBCL cells include Sudhl4 and Sudhl8 cells, all purchased from Shanghai Chinese Academy of Sciences Cell Place.

[0031] ...

Embodiment 2

[0044] Effect of isoliensinine on apoptosis induction of leukemia cell lines

[0045] 2.1. Experimental materials

[0046] 2.1.1 Reagents and materials

[0047] ① compound

[0048] Same as 1.1.1.

[0049] ② cell line

[0050] Human T cell malignant tumor cells Jurkat were purchased from the Institute of Cells, Chinese Academy of Sciences, Shanghai.

[0051] ③ Cell culture reagents

[0052] Same as 1.1.1.

[0053] ④ Relevant index detection kits

[0054] Annexin V / PI Cell Apoptosis Detection Kit (Novizan Biotechnology, Nanjing, China) was used to detect cell apoptosis.

[0055] 2.1.2 Experimental Instruments

[0056] YJ-875 type medical purification bench (purification equipment factory, Suzhou, China); 3111 type water jacket CO 2 Incubator (ThermoFisher Scientific, Waltham, MA, USA); 702 type ultra-low temperature refrigerator (ThermoFisher Scientific); electronic balance (Sartorius Instrument Systems Co., Ltd., Beijing, China); QIUJING blood cell counter (Qiujing Bio...

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PUM

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Abstract

The invention discloses an application of isoliensinine in preparation of drugs for treating leukemia, the structure of isoliensinine is as shown in formula (1), and the leukemia comprises acute myelogenous leukemia, chronic granulocytic leukemia, T-cell malignant tumor and diffuse large B-cell lymphoma.

Description

technical field [0001] The invention relates to an application of a compound, and discloses the application of isoliensinine and a pharmaceutically acceptable salt thereof in the preparation of drugs for preventing and / or treating leukemia. Background technique [0002] Lianzixin is the green germ in the middle of the mature seeds of Nelumbo nuciferia Gaertn, a plant of the family Nymphaeaceae. It is a traditional Chinese medicine in clinical practice and one of the important medicinal and edible crops. It has the function of clearing away heat and detoxifying, and is used for fever. Isoliensinine (English name: isoliensinine; abbreviation: IsoL) is one of the main components of lotus alkaloids. It has been proven to have various effects such as lowering blood pressure and antiarrhythmia. Antiarrhythmic Mechanism of Antiarrhythmic Mechanism" found that isoliensinine plays an antiarrhythmic role by inhibiting the front sodium current, L-type calcium current and late sodium cu...

Claims

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Application Information

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IPC IPC(8): A61K31/4725A61K36/62A61P35/02A61P35/00
CPCA61K31/4725A61K36/62A61P35/02A61P35/00Y02P20/55
Inventor 胡珀潘扬曹鹏王歆竹杨光明陈景
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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