Automatic high-throughput PCR (Polymerase Chain Reaction) portable equipment based on chip

Abstract

The invention discloses chip-based automatic high-flux PCR portable equipment, which comprises a thermal cycler, a template cabin is fixedly mounted on the front side of the thermal cycler, a plurality of groups of delivery pumps are connected to the upper end of the template cabin through pipelines, a plurality of groups of feeding pipes are fixedly connected to the upper end of the thermal cycler, a plurality of groups of sample chambers are arranged on the inner side of the thermal cycler, and the sample chambers are communicated with the template cabin through pipelines. The multiple groups of feeding pipes are in one-to-one correspondence with the sample chambers, adding devices are fixedly mounted at the upper ends of the multiple groups of feeding pipes, reagent cabins are connected to the upper ends of the multiple groups of adding devices through pipelines, the conveying pump is connected to the adding devices through pipelines, and a control chip is arranged on the inner side of the thermal cycler. A touch screen is fixedly installed on the front side of the template cabin, a temperature control device is arranged on the lower portion of the inner side of the thermal cycler and electrically connected with a control chip, and multiple sets of template grooves are formed in the inner side of the template cabin.

Description

technical field [0001] The invention is applied to the technical background of molecular biology, and the name is a chip-based automatic high-throughput PCR portable device. Background technique [0002] Polymerase chain reaction (PCR) is a technique for replicating specific DNA sequences, three basic steps in the process: splitting the DNA template into two single strands (denaturing), adding short pieces of complementary DNA called primers, to initiate replication of the selected DNA sequence (annealing), and to add DNA polymerase to synthesize the complementary strand (extension), repeated again and again to amplify the sequence, each of these steps at different temperatures at optimal Therefore, heating and cooling are performed using an instrument called a thermal cycler. The PCR thermal cycler is currently commercialized and relatively mature, but there are still some problems, such as: the instrument throughput is low and cannot meet high-throughput The demand for sa...

AI Technical Summary

Problems solved by technology

[0002] Polymerase chain reaction (PCR) is a technique for replicating specific DNA sequences, three basic steps in the process: splitting the DNA template into two single strands (denaturing), adding short pieces of complementary DNA called primers, to initiate replication of the selected DNA sequence (annealing), and to add DNA polymerase to synthesize the complementary strand (extension), repeated again and again to amplify the sequence, each of these steps at different temperatures at optimal Therefore, heating and cooling are performed using an instrument called a thermal cycler. The PCR thermal cycler is currently commercialized and relatively mature, but there are still some problems, such as: the instrument throughput is low and cannot meet high-throughput The demand for samples can only be met by increasing the number of instruments; the instruments are relatively large, and it is difficult to achieve portability and safety; for chemical / radioactive reagents, it is easy to cause environmental pollution and sample waste; relying on manual operation, it is difficult to achieve automation; The requirements are high, and a series of experimental operations and temperature settings need to be completed; therefore, it is necessary to provide a chip-based automated high-throughput PCR portable device that can achieve the role of automation

Images