Walnut meal polyphenol with lipid-lowering effect as well as preparation method and application of walnut meal polyphenol

A technology for walnut meal and hyperlipidemia, which is applied in the field of biological utilization, can solve problems such as astringency, development and utilization restrictions, etc., and achieve the effects of removing astringency, expanding resource utilization, and improving product quality.

Pending Publication Date: 2022-04-29
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Walnut meal is rich in protein and is a high-quality vegetable protein raw material, but its development and utilization are lim

Method used

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  • Walnut meal polyphenol with lipid-lowering effect as well as preparation method and application of walnut meal polyphenol
  • Walnut meal polyphenol with lipid-lowering effect as well as preparation method and application of walnut meal polyphenol
  • Walnut meal polyphenol with lipid-lowering effect as well as preparation method and application of walnut meal polyphenol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Preparation of walnut dregs polyphenols with lipid-lowering effect: mechanically pulverize walnut dregs after simple impurity removal pretreatment, and pass through a 60-mesh sieve; add 15% ethanol solution to walnut dregs after crushing and sieving, The liquid ratio is 1:50 (g / mL), and then reflux extraction is carried out. The extraction conditions are: extraction temperature 60°C, extraction time 60 min. Vacuum filtration is used to separate the solid from the liquid, the solid is deastringent walnut dregs, and the liquid is walnut dreg polyphenols; the solid particles and the liquid after removing the solid particles are taken respectively; the liquid after removing the solid particles is processed by a rotary evaporator Concentrating; preparing the concentrated solution through freeze-drying technology to obtain polyphenols from walnut dregs. The polyphenol yield of walnut meal was 4.61%.

Embodiment 2

[0037] Preparation of walnut dregs polyphenols with lipid-lowering effect: mechanically pulverize walnut dregs after simple impurity removal pretreatment, and pass through a 60-mesh sieve; add 85% ethanol solution to walnut dregs after crushing and sieving, The liquid ratio is 1:50 (g / mL), and then reflux extraction is carried out. The extraction conditions are: extraction temperature 60°C, extraction time 60 min. Vacuum filtration is used to separate the solid from the liquid, the solid is deastringent walnut dregs, and the liquid is walnut dreg polyphenols; the solid particles and the liquid after removing the solid particles are taken respectively; the liquid after removing the solid particles is processed by a rotary evaporator Concentrating; preparing the concentrated solution through freeze-drying technology to obtain polyphenols from walnut dregs. The polyphenol yield of walnut meal was 4.36%.

Embodiment 3

[0039] Preparation of walnut dregs polyphenols with lipid-lowering effect: walnut dregs are mechanically pulverized after simple impurity removal pretreatment, and passed through a 60-mesh sieve; optimal extraction conditions are designed and optimized by response surface methodology, and then crushed and sieved Add 50% ethanol solution to the final walnut meal, the ratio of solid to liquid is 1:50 (g / mL), and then carry out reflux extraction. Extraction conditions: extraction temperature 73°C, extraction time 60min. Vacuum filtration is used to separate the solid from the liquid, the solid is deastringent walnut dregs, and the liquid is walnut dreg polyphenols; the solid particles and the liquid after removing the solid particles are taken respectively; the liquid after removing the solid particles is processed by a rotary evaporator Concentrating; preparing the concentrated solution through freeze-drying technology to obtain polyphenols from walnut dregs. The polyphenol yiel...

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Abstract

The invention discloses walnut meal polyphenol with a lipid-lowering function as well as a preparation method and application of the walnut meal polyphenol. The walnut meal polyphenol has the effects of lowering triglyceride (TG), total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) of hyperlipemia serum and liver; the walnut dreg polyphenol is prepared from the following components in percentage by weight: 16.02% of Glansginin A, 15.60% of gallic acid, 12.53% of ellagic acid, 6.84% of ellagic acid-4-O-xyloside, 5.91% of neofusasin I, 4.42% of ethyl gallate, 3.73% of isoburning fat, 2.46% of galloylglucose, 1.08% of digalloylglucose and 1.04% of erythrosine. According to the preparation method, the extraction yield of the walnut meal polyphenol is 4.93%-6.95%. According to the method, a response surface design reflux extraction technology is adopted and is combined with the reflux extraction technology, so that the extraction rate of the walnut meal polyphenol is greatly improved, and the extraction period is effectively shortened; the preparation method disclosed by the invention is simple and convenient, easy to operate, short in time consumption and low in cost, can realize rapid deastringency of the walnut meal, can efficiently produce the functional component walnut meal polyphenol, and effectively expands resource development and utilization of the walnut meal.

Description

technical field [0001] The invention belongs to the technical field of bioutilization, and in particular relates to a method for reducing serum triglyceride, total cholesterol, low-density lipoprotein cholesterol (LDL-C), liver triglyceride (TG), and total cholesterol (TC) in hyperlipidemia. 1. Walnut meal polyphenols with the effect of low-density lipoprotein cholesterol (LDL-C) and its preparation method and application. Background technique [0002] Walnut( Juglans regin Linn ) belongs to the Juglandaceae plant, mainly distributed in Asia, Europe, America and other continents. Walnut has a long history of cultivation in my country and is rich in resources. The planting area and output of walnuts in my country rank first in the world, and Yunnan ranks first in the country. In addition to being eaten directly, walnuts are mainly used as oil for the production of walnut oil and walnut milk. The by-products of walnut meal after walnut oil extraction are rich in nutrients ...

Claims

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Application Information

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IPC IPC(8): A61K36/52A61P3/06A23L33/105A23C11/10A23L33/18
CPCA61K36/52A61P3/06A23L33/105A23C11/103A23L33/18A61K2236/333A61K2236/55A23V2002/00A23V2250/2132A23V2200/30A23V2200/3262A23V2300/14A23V2300/02
Inventor 赵声兰和兴萍陈丹赵庆宇婧林玉萍马雅鸽张希
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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