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Excipient of microbial culture medium, microbial culture medium, application of microbial culture medium and microbial culture bottle

A microbial culture medium and microbial culture technology, applied in the field of microbial culture, can solve problems such as affecting the accuracy of experiments, inability to observe, and being too viscous, and achieve the effects of stable molding, easy observation, and good gel formation.

Inactive Publication Date: 2022-04-12
许睿
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

High-concentration starch excipient culture medium is similar to paste, opaque and too viscous. When screening for specific microorganisms, starch is also used as a chromogenic substrate, and the pigment produced close to the test tube wall can be observed. Pigments are difficult or impossible to observe, which affects the accuracy of the experiment

Method used

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  • Excipient of microbial culture medium, microbial culture medium, application of microbial culture medium and microbial culture bottle
  • Excipient of microbial culture medium, microbial culture medium, application of microbial culture medium and microbial culture bottle
  • Excipient of microbial culture medium, microbial culture medium, application of microbial culture medium and microbial culture bottle

Examples

Experimental program
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Effect test

Embodiment 1

[0067] The microorganism culture medium of the application embodiment comprises following metering components:

[0068]

[0069] After autoclaving according to the preparation requirements of the microbial culture medium, adjust the pH to 7.5±0.1.

Embodiment 2

[0071] The microorganism culture medium of the application embodiment comprises following metering components:

[0072]

[0073]

[0074] After autoclaving according to the preparation requirements of the microbial culture medium, adjust the pH to 7.5±0.1.

Embodiment 3

[0076] The microorganism culture medium of the application embodiment comprises following metering components:

[0077]

[0078] After autoclaving according to the preparation requirements of the microbial culture medium, adjust the pH to 7.5±0.1.

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Abstract

The invention provides an excipient of a microbial culture medium, the microbial culture medium, application of the microbial culture medium and a microbial culture bottle. The excipient of the microbial culture medium comprises one or a combination of at least two of vegetable gum and microbial gum, the prepared microbial culture medium is transparent or semitransparent, the growth condition of bacteria in the culture medium can be clearly observed, the size of a three-dimensional space formed in the culture medium can be controlled, free flowing of nutrient substances can be guaranteed, and the growth condition of bacteria in the culture medium can be ensured. The target microorganisms can grow in a positioned manner to facilitate observation, and the target microorganisms with power can penetrate through the device, so that the purpose of separation is achieved.

Description

technical field [0001] The application belongs to the technical field of microorganism cultivation, and more specifically, relates to an excipient of a microorganism culture medium, a microorganism culture medium and its application, and a microorganism culture bottle. Background technique [0002] Microbial culture medium is a basic tool for studying microorganisms. According to common containers, it can be divided into plate / plate type, tube type, and bottle type; according to physical form, it can be divided into liquid, semi-solid, and solid. Among them, semi-solid and solid microbial culture media need Add excipients to meet the use requirements, but different microorganisms have different growth characteristics. In order to study the optimal isolation, growth and observation conditions of some specific microorganisms, the choice of excipients is also one of the improvements. [0003] Most of the excipients used in the existing microbial culture medium are agar, and gel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/04C12M1/24C12R1/46
Inventor 许睿
Owner 许睿
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